PMID: 9554075Apr 29, 1998Paper

Genetic heterogeneity and PCR detection of Cryptosporidium parvum

Advances in Parasitology
Giovanni Widmer

Abstract

A variety of methods have been applied to the study of genotypic and phenotypic polymorphism in Cryptosporidium parvum. Results from these studies have consistently shown the existence of different genotypes and phenotypes within the species. A long-term goal of this work is the identification of markers for virulence in humans and animals and the elucidation of transmission cycles of C. parvum. Achievement of these goals will depend on the identification of highly polymorphic loci. Of particular interest are polymorphisms amenable to typing by polymerase chain reaction (PCR), as C. parvum cannot be expanded in vitro. Fingerprinting of isolates by restriction of PCR fragments or allele-specific PCR has given promising results. As originally observed by isoenzyme analysis, genetic fingerprinting has confirmed the occurrence in humans of unique C. parvum genotypes which are not found among calf isolates. This observation remains to be reconciled with the cross-infectivity of C. parvum to ruminant and nonruminant hosts and the important role that bovines play in the epidemiology of C. parvum and human cryptosporidiosis. Although PCR detection of C. parvum DNA from individual oocysts has been reported, the sensitivity of PCR detect...Continue Reading

Citations

Nov 4, 2008·Parasitology Research·Annalisa LeoneGuido Maria Grasso
Nov 27, 1999·International Journal for Parasitology·D C de GraafJ E Peeters
Oct 11, 2003·Molecular and Biochemical Parasitology·Sultan TanriverdiGiovanni Widmer
May 9, 2000·Veterinary Parasitology·C A Vergara-CastiblancoM E Ares-Mazás
Aug 24, 1999·Veterinary Parasitology·R B Gasser
Nov 1, 2001·Diagnostic Microbiology and Infectious Disease·L A Ward, Y Wang
Aug 23, 2002·Microbes and Infection·Saul Tzipori, Honorine Ward
Aug 23, 2002·Microbes and Infection·Giovanni WidmerMitchell S Abrahamsen
Aug 5, 2000·Applied and Environmental Microbiology·X FengG Widmer
Mar 7, 2001·Applied and Environmental Microbiology·J F Perz, S M Le Blancq
Jan 17, 2004·Clinical Microbiology Reviews·Lihua XiaoSteve J Upton
Sep 23, 2003·Infection and Immunity·Donna E AkiyoshiSaul Tzipori
Aug 31, 2002·Journal of Clinical Microbiology·Sultan TanriverdiGiovanni Widmer
Mar 8, 2003·Journal of Clinical Microbiology·Umaporn ThathaisongSaovanee Leelayoova
Dec 1, 1999·International Journal for Parasitology·R B Gasser, P O'Donoghue
Mar 31, 2016·International Journal for Parasitology·Alex Grinberg, Giovanni Widmer
Sep 19, 2006·Water Research·Nathalie TufenkjiCalum J Drummond
Feb 11, 2015·Experimental Parasitology·Marzieh Ezzaty MirhashemiTheo De Waal
May 26, 1999·Emerging Infectious Diseases·N J PieniazekD G Addiss
Mar 4, 2006·The Korean Journal of Parasitology·Jae-Hwan ParkJong-Yil Chai
Apr 26, 2000·The Journal of Parasitology·N V KhramtsovS J Upton
Jan 19, 2002·Annals of the New York Academy of Sciences·J S KoopmanS E Chick
Apr 2, 1999·Applied and Environmental Microbiology·G WidmerS Tzipori
Oct 9, 1999·Clinical Microbiology Reviews·D P Clark

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