Genetic heterozygosity in unreplicated bacteriophage lambda recombinants.

Genetics
R L White, M S Fox

Abstract

Bacteriophage crosses using density-labeled parents have been carried out under conditions restricting DNA synthesis. The parental material and genetic contributions to progeny manifesting recombination within a genetic interval sufficiently short to exhibit high negative interference have been examined. The unreplicated products of recombination isolated as phage particles appear to contain long continuous heteroduplex regions which are heterozygous for the closely linked markers. Recombination between closely linked markers seems to be the consequence of the removal of base-pair mismatches that are present within the heteroduplex regions. This localized reduction of heterozygosity within the heteroduplex regions that join the parental components of recombinant DNA molecules can account for high negative interference.

Citations

Jun 5, 1979·Journal of Molecular Biology·E J Sodergren, M S Fox
Nov 24, 1975·Molecular & General Genetics : MGG·R L White, M S Fox
Jan 20, 1985·Journal of Molecular Biology·F W StahlM M Stahl
Oct 13, 2016·Annual Review of Genetics·James E Haber

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.