Genetic identification of Entamoeba polecki subtype 3 from pigs in Japan and characterisation of its pathogenic role in ulcerative colitis

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
Makoto MatsubayashiTomoyuki Shibahara

Abstract

To date, three Entamoeba spp. (E. suis, zoonotic E. polecki and E. histolytica) have been identified in pigs, but their pathogenicity and molecular classification have not been fully determined. Examination and pathological analysis of pigs (n=3) with diarrhoea was conducted and revealed the presence of Entamoeba organisms. We performed a genetic analysis of the isolate using the small-subunit ribosomal RNA (SSU rRNA) gene region to identify the species. A severe ulcerative colitis was observed histopathologically with inflammatory cells, including macrophages and neutrophils, infiltrating the mucous membranes of the cecum and colon. Many Entamoeba trophozoites were found at the erosion site or at ulcerative lesions. Pathogenic viruses or bacteria were not detected. The SSU rRNA sequence of the Entamoeba isolate was found to be completely homologous to that of E. polecki subtype 3.

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Citations

Dec 1, 2017·Parasitology Research·Makoto MatsubayashiKazumi Sasai
Feb 12, 2019·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Maria Cuvertoret-SanzJoaquim Segalés
Nov 30, 2019·BioMed Research International·Ting JiDong-Hui Zhou
Mar 2, 2018·Journal of Clinical Microbiology·Christen Rune StensvoldMarianne Lebbad
Feb 27, 2020·The Journal of Veterinary Medical Science·Hirotaka ItoTomoyuki Shibahara
Jul 21, 2020·The Journal of Veterinary Medical Science·Tetsuya KomatsuTomoyuki Shibahara
May 23, 2021·Veterinary Medicine and Science·Roshan B AdhikariTirth R Ghimire

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