Genetic immunization against hepatitis B virus with calcium phosphate nanoparticles in vitro and in vivo

Acta Biomaterialia
Leonardo Rojas-SánchezMatthias Epple

Abstract

Calcium phosphate nanoparticles were loaded with plasmid DNA and toll-like receptor ligands (TLR), i.e. CpG or flagellin, to activate antigen-presenting cells (APCs) like dendritic cells (DCs). The functionalized nanoparticles were studied in vitro on HeLa, C2C12 and BHK-21 cell lines, focusing on the expression of two specific proteins. EGFP-DNA, encoding for enhanced green fluorescent protein (EGFP), was used as a model plasmid to optimize the transfection efficiency in vitro by fluorescence microscopy and flow cytometry. Calcium phosphate nanoparticles loaded with TLR ligands and plasmid DNA encoding for the hepatitis B virus surface antigen (pHBsAg) were evaluated by in vitro and in vivo immunization experiments to identify a possible candidate for a prophylactic hepatitis B virus (HBV) vaccine. The nanoparticles induced a strong expression of HBsAg in the three cell lines. In splenocytes, the expression of the co-stimulatory molecules CD80 and CD86 was enhanced. After intramuscular injection in mice, the nanoparticles induced the expression of HBsAg, the antigen-specific T cell response, and the antigen-specific antibody response (IgG1). STATEMENT OF SIGNIFICANCE: Hepatitis B is one of the most frequent viral infections wo...Continue Reading

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Citations

Feb 13, 2021·Chemistry : a European Journal·Viktoriya Sokolova, Matthias Epple
Apr 21, 2021·Journal of Inorganic Biochemistry·Lichun MaoChunying Chen
Nov 28, 2021·Nanoscale Research Letters·Sagar RayamajhiRobert DeLong

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