Genetic incorporation of D-lysine into diketoreductase in Escherichia coli cells

Amino Acids
Zhizhi LiuYijun Chen

Abstract

Pyrococcus horikoshii lysyl-tRNA synthetase/tRNA orthogonal pair exhibited high selectivity towards D-lysine in the presence of excess amount of D-lysine. Based on the observation, this orthogonal pair was employed to encode D-lysine, and D-lysine was site-specifically incorporated into the diketoreductase in E. coli cells.

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