Genetic integration in the heterospecific transformation of Haemophilus influenzae cells by Haemophilus parainfluenzae deoxyribonucleic acid.

Journal of Bacteriology
W L Steinhart, R M Herriott

Abstract

The in vivo chemical linkage of Haemophilus parainfluenzae deoxyribonucleic acid (DNA) with the H. influenzae genome has been found to occur at a much higher level than is suggested by the low efficiency of the heterospecific transformation of an antibiotic resistance marker. This linkage, about 60% of the level with homospecific DNA, was found to involve alkali-stable bonding. The amount of host DNA label released (about 60%) was about the same as that released during homospecific transformation. Also, over 60% of the H. influenzae cells adsorbing H. parainfluenzae DNA could not form colonies upon plating. This lethality of the heterospecific transformation was not immediate but followed considerable metabolic activity of the host cells. These data are presented to show that the "limited-pairing" hypothesis may be only a partial explanation for the low efficiency of heterospecific transformation. Another hypothesis is presented which takes into account the lethal effect of this kind of transformation.

Citations

Jan 1, 1984·Molecular & General Genetics : MGG·W L AlbrittonA G Steigerwalt
Nov 26, 2002·Journal of Bacteriology·Bryan J WilliamsArnold L Smith
Jan 1, 1984·The Journal of Membrane Biology·M E Kahn, H O Smith
Jan 5, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Derek K HoArnold L Smith
Jul 2, 2004·Molecular Microbiology·Michael E WatsonArnold L Smith
Jun 9, 1971·Nature: New Biology·K L Beattie, J K Setlow
Feb 27, 2014·G3 : Genes - Genomes - Genetics·Joshua Chang MellRosemary J Redfield
Aug 19, 2009·Infection and Immunity·Roma KenjaleJoseph W St Geme
Oct 1, 1970·Journal of Bacteriology·K L Beattie, J K Setlow
Dec 1, 1981·Journal of Bacteriology·N K NotaniN L Clayton
Jul 1, 1973·Journal of Bacteriology·J K SetlowK L Beattie
May 1, 1974·Journal of Bacteriology·J J ScoccaK C Zoon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Molecular & General Genetics : MGG
W L AlbrittonA G Steigerwalt
Medicinski Glasnik : Official Publication of the Medical Association of Zenica-Doboj Canton, Bosnia and Herzegovina
Vladimira LeskovarJasmina Vranes
© 2021 Meta ULC. All rights reserved