Genetic interactions between TFIIF and TFIIS.

Genetics
Rachel N FishCaroline M Kane

Abstract

The eukaryotic transcript elongation factor TFIIS is encoded by a nonessential gene, PPR2, in Saccharomyces cerevisiae. Disruptions of PPR2 are lethal in conjunction with a disruption in the nonessential gene TAF14/TFG3. While investigating which of the Taf14p-containing complexes may be responsible for the synthetic lethality between ppr2Delta and taf14Delta, we discovered genetic interactions between PPR2 and both TFG1 and TFG2 encoding the two larger subunits of the TFIIF complex that also contains Taf14p. Mutant alleles of tfg1 or tfg2 that render cells cold sensitive have improved growth at low temperature in the absence of TFIIS. Remarkably, the amino-terminal 130 amino acids of TFIIS, which are dispensable for the known in vitro and in vivo activities of TFIIS, are required to complement the lethality in taf14Delta ppr2Delta cells. Analyses of deletion and chimeric gene constructs of PPR2 implicate contributions by different regions of this N-terminal domain. No strong common phenotypes were identified for the ppr2Delta and taf14Delta strains, implying that the proteins are not functionally redundant. Instead, the absence of Taf14p in the cell appears to create a dependence on an undefined function of TFIIS mediated by i...Continue Reading

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Citations

Feb 25, 2010·Molecular Genetics and Genomics : MGG·Julia M SchulzeAna Ruiz-Manzano
May 31, 2007·Journal of Biosciences·Parag SadhaleAruna Naorem
Mar 26, 2008·Molecular and Cellular Biology·Denys A KhaperskyyAlfred S Ponticelli
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Jan 20, 2009·Journal of Molecular Biology·Marion GrasserKlaus D Grasser
Mar 18, 2015·Molecular and Cellular Biology·Arnob DuttaJoseph C Reese

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