Genetic labelling of specific axonal pathways in the mouse central nervous system

The European Journal of Neuroscience
Mohamad ZubairMasaharu Noda

Abstract

We report on transgenic mouse lines in which several sensory systems in the brain are specifically visualized genetically. We employed GAP-lacZ as an axon-targeted reporter protein that was constructed by fusing the membrane-anchoring domain of the GAP-43 protein to lacZ. The reporter gene was introduced into the genome under the control of a promoter element of Brn3b transcription factor to establish transgenic mouse lines. The individual lines thus generated afforded clear images of specific axonal pathways of the visual, vomeronasal, pontocerebellar, and auditory systems. The reporter protein labelled the entire axonal process as well as the cell body of developing and mature neurons on staining with X-gal. We show that these lines facilitate the developmental and anatomical study of these neural systems. This strategy should be applicable to a variety of neural systems by using various specific promoter elements.

References

Mar 22, 1985·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·U C Dräger
Feb 15, 1988·The Journal of Comparative Neurology·R E Wickesberg, D Oertel
Apr 20, 1981·The Journal of Comparative Neurology·S A AziziD J Woodward
Jun 1, 1980·The Journal of Comparative Neurology·U C Dräger, J F Olsen
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·C A Callahan, J B Thomas
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M R GerreroM G Rosenfeld
Nov 15, 1996·Cell·P MombaertsR Axel

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