Genetic manipulation of Campylobacter jejuni.

Current Protocols in Microbiology
Lindsay DavisVictor DiRita

Abstract

Molecular manipulation has been a limiting factor in C. jejuni research for many years. Recent advances in molecular techniques adapted for C. jejuni have furthered our understanding of the organism. This unit is dedicated to common molecular tools in bacterial research specifically tailored for C. jejuni. These include colony PCR, DNA isolation, and RNA isolation. The unit also reviews techniques for genetic manipulation, such as the use of plasmids, natural transformation, electroporation, conjugation, and transposition. In addition, a reporter system, the arylsulfatase assay, can be used to study gene expression.

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Citations

Apr 26, 2015·Applied and Environmental Microbiology·Ximin ZengJun Lin
Aug 21, 2010·Journal of Applied Microbiology·I I KassemG Rajashekara
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May 16, 2021·Applied and Environmental Microbiology·Luyao MaXiaonan Lu
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