Genetic Manipulation of Cryptococcus neoformans

Current Protocols in Microbiology
Kwang-Woo JungYong-Sun Bahn

Abstract

Cryptococcus neoformans is an opportunistic fungal pathogen, which causes life-threatening meningoencephalitis in immunocompromised individuals and is responsible for more than 1,000,000 infections and 600,000 deaths annually worldwide. Nevertheless, anti-cryptococcal therapeutic options are limited, mainly because of the similarity between fungal and human cellular structures. Owing to advances in genetic and molecular techniques and bioinformatics in the past decade, C. neoformans, belonging to the phylum basidiomycota, is now a major pathogenic fungal model system. In particular, genetic manipulation is the first step in the identification and characterization of the function of genes for understanding the mechanisms underlying the pathogenicity of C. neoformans. This unit describes protocols for constructing target gene deletion mutants using double-joint (DJ) PCR, constitutive overexpression strains using the histone H3 gene promoter, and epitope/fluorescence protein-tagged strains in C. neoformans. © 2018 by John Wiley & Sons, Inc.

References

Mar 1, 1992·Infection and Immunity·A VarmaK J Kwon-Chung
Sep 1, 1991·Infection and Immunity·D R Kirsch, R R Whitney
Apr 1, 1991·Journal of Clinical Microbiology·A Varma, K J Kwon-Chung
Feb 1, 1990·Current Genetics·D ArmaleoS A Johnston
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T L Orr-WeaverR J Rothstein
Mar 1, 1993·Journal of Bacteriology·D L ToffalettiJ R Perfect
Jun 1, 1995·Molecular Microbiology·B L Wickes, J C Edman
Nov 1, 1996·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·G M CoxJ R Perfect
Jan 5, 2000·Clinical and Diagnostic Laboratory Immunology·J HuaJ K Lodge
Apr 12, 2000·International Journal of Systematic and Evolutionary Microbiology·G CardinaliA Martini
Apr 26, 2000·Fungal Genetics and Biology : FG & B·R C DavidsonJ Heitman
Nov 22, 2000·Mycopathologia·J HeitmanJ R Perfect
Mar 29, 2001·Medical Mycology·H C McDade, G M Cox
Jan 30, 2003·Fungal Genetics and Biology : FG & B·Rex T NelsonJennifer K Lodge
Sep 1, 2005·Nature Reviews. Microbiology·Alexander IdnurmJoseph Heitman
Feb 25, 2006·Fungal Genetics and Biology : FG & B·J FuB L Wickes
May 18, 2006·Annual Review of Microbiology·Xiaorong Lin, Joseph Heitman
Oct 28, 2008·Fungal Genetics and Biology : FG & B·Jack A RuffLorina G Baker
Oct 27, 2009·Biochemical and Biophysical Research Communications·Min Su KimYong-Sun Bahn
Feb 23, 2010·PLoS Pathogens·Teresa R O'MearaJ Andrew Alspaugh
Jun 21, 2011·Microbes and Infection·Edmond J ByrnesJoseph Heitman
Oct 12, 2011·Immunological Investigations·Michael S Price, John R Perfect
Oct 9, 2012·Eukaryotic Cell·Dong-Hoon YangYong-Sun Bahn
Apr 8, 2015·Nature Communications·Kwang-Woo JungYong-Sun Bahn
Sep 30, 2016·Nature Communications·Kyung-Tae LeeYong-Sun Bahn

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Citations

Jan 21, 2019·Current Protocols in Microbiology·Sheng SunJoseph Heitman
Feb 26, 2019·Current Protocols in Microbiology·Andrew L ChangTamara L Doering
Sep 30, 2020·The Journal of Microbiology·Kwang-Woo JungYong-Sun Bahn
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kwang-Woo JungSungbeom Lee

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