Genetic manipulation of reptilian embryos: toward an understanding of cortical development and evolution

Frontiers in Neuroscience
Tadashi NomuraKatsuhiko Ono

Abstract

The mammalian neocortex is a remarkable structure that is characterized by tangential surface expansion and six-layered lamination. However, how the mammalian neocortex emerged during evolution remains elusive. Because all modern reptiles have a homolog of the neocortex at the dorsal pallium, developmental analyses of the reptilian cortex are valuable to explore the origin of the neocortex. However, reptilian cortical development and the underlying molecular mechanisms remain unclear, mainly due to technical difficulties with sample collection and embryonic manipulation. Here, we introduce a method of embryonic manipulations for the Madagascar ground gecko and Chinese softshell turtle. We established in ovo electroporation and an ex ovo culture system to address neural stem cell dynamics, neuronal differentiation and migration. Applications of these techniques illuminate the developmental mechanisms underlying reptilian corticogenesis, which provides significant insight into the evolutionary steps of different types of cortex and the origin of the mammalian neocortex.

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Citations

Oct 20, 2015·Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis·Zuzana Starostová, Zuzana Musilová
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Methods Mentioned

BETA
dissecting
transfection

Software Mentioned

Ensemble Genome Browser

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