Genetic mapping of psl locus and quantitative trait loci for angular leaf spot resistance in cucumber (Cucumis sativus L.)

Molecular Breeding : New Strategies in Plant Improvement
Renata SłomnickaGrzegorz Bartoszewski

Abstract

One of the most important cucumber diseases is bacterial angular leaf spot (ALS), whose increased occurrence in open-field production has been observed over the last years. To map ALS resistance genes, a recombinant inbred line (RIL) mapping population was developed from a narrow cross of cucumber line Gy14 carrying psl resistance gene and susceptible B10 line. Parental lines and RILs were tested under growth chamber conditions as well as in the field for angular leaf spot symptoms. Based on simple sequence repeat and DArTseq, genotyping a genetic map was constructed, which contained 717 loci in seven linkage groups, spanning 599.7 cM with 0.84 cM on average between markers. Monogenic inheritance of the lack of chlorotic halo around the lesions, which is typical for ALS resistance and related with the presence of recessive psl resistance gene, was confirmed. The psl locus was mapped on cucumber chromosome 5. Two major quantitative trait loci (QTL) psl5.1 and psl5.2 related to disease severity were found and located next to each other on chromosome 5; moreover, psl5.1 was co-located with psl locus. Identified QTL were validated in the field experiment. Constructed genetic map and markers linked to ALS resistance loci are novel r...Continue Reading

References

May 16, 2002·The Journal of Heredity·R E Voorrips
Jun 12, 2004·Proceedings of the National Academy of Sciences of the United States of America·Peter WenzlAndrzej Kilian
Oct 22, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Y SakataM Hirai
Nov 3, 2009·Nature Genetics·Sanwen HuangSonggang Li
Apr 11, 2012·The Plant Journal : for Cell and Molecular Biology·Luming YangYiqun Weng
May 22, 2013·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Xiaoming HeYiqun Weng
Nov 15, 2015·Plant Science : an International Journal of Experimental Plant Biology·Magdalena PawełkowiczZbigniew Przybecki
May 6, 2016·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yuhui WangYiqun Weng
Oct 8, 2016·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Khin Thanda WinSanghyeob Lee
Nov 22, 2017·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yuhui WangYiqun Weng

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Methods Mentioned

BETA
genotyping
electrophoresis

Software Mentioned

DArTseq
MapChart
MapQTL
InterProScan
Joinmap
Blast2GO

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