PMID: 6987491Jan 1, 1980Paper

Genetic markers for quantitative mutagenesis studies in Chinese hamster ovary cells: characteristics of some recently developed selective systems

Mutation Research
R S Gupta, L Siminovitch

Abstract

Selection conditions have been optimized in the Chinese hamster ovary (CHO) cell system for a number of genetic markers. The genetic systems studied include resistance to the protein-synthesis inhibitors emetine (Emtr) and diphtheria toxin (Dipr), resistance to methylglyoxalbisguanylhydrazone (Mbgr) which affects polyamine transport, resistance to the nucleoside analogs toyocamycin and tubercidin (Toyr), and resistance to thioguanine (Thgr) and ouabain (OuaR). The optimal expression time following mutagenesis for various markers was between 2 and 6 days. A linear dose--response relationship between the concentration of mutagen (ethyl methanesulfonate) and mutation frequency has been observed over the range of 10--700 micrograms/ml, for all of the above markers except Toyr. The response of these markers to other mutagens such as tritium (3H) decay and ICR-191 show some specificity. Since the response of a number of genetic markers can be studied simultaneously in the CHO system, it should prove very useful for studies of quantitative mutagenesis and in assay systems for mutagen detection.

References

Oct 1, 1975·Experimental Cell Research·C M Corsaro, B R Migeon
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·U Friedrich, P Coffino
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R S Gupta, L Siminovitch
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C WaldrenT T Puck
Jan 1, 1977·Somatic Cell Genetics·R G WortonC Duff
Dec 1, 1978·Journal of Cellular Physiology·J L Mandel, W F Flintoff
Jan 1, 1976·Annual Review of Biochemistry·J W Drake, R H Baltz
Oct 1, 1977·Nature·J P O'Neill, A W Hsie
Oct 1, 1976·Archives of Biochemistry and Biophysics·K H Astrin, C T Caskey
Jan 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·E Huberman, L Sachs
Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·A L BeaudetC T Caskey
Aug 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·B N AmesF D Lee
Nov 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J D SharpM R Capecchi
Oct 1, 1973·Journal of Cellular Physiology·L A Chasin
Mar 10, 1971·Nature: New Biology·C P StannersH Green

❮ Previous
Next ❯

Citations

Mar 1, 1986·Somatic Cell and Molecular Genetics·G M Adair, M J Siciliano
Jul 1, 1986·Somatic Cell and Molecular Genetics·A Chopra, R S Gupta
Aug 1, 1982·Mutation Research·A A MorleyJ L Dempsey
Sep 1, 1983·Mutation Research·J ThackerM B Webb
Dec 1, 1984·Mutation Research·H P Leenhouts, K H Chadwick
Mar 1, 1991·Mutation Research·H U Aeschbacher, R J Turesky
Jun 1, 1983·Toxicology Letters·S Takayama, M Tanaka
Jun 19, 2001·Mutation Research·P J DonovanC W Riggs
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·B L Allen-Hoffmann, J G Rheinwald
Aug 1, 1983·European Journal of Biochemistry·K B FreemanM Buckle
Jun 30, 1980·Biochemical and Biophysical Research Communications·R S Gupta
May 1, 1980·Journal of Cellular Physiology·R S Gupta
Dec 2, 2005·Molecular Biology of the Cell·Nica M BorradaileJean E Schaffer
Feb 1, 1983·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·R D WoodA Poley
Jun 1, 1986·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·J C FuscoeM Fox

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.