PMID: 8971275Sep 1, 1996Paper

Genetic markers in ribosomal DNA for hookworm identification

Acta Tropica
Robin B GasserR Speare

Abstract

Polymerase chain reaction-linked restriction fragment length polymorphism (PCR-RFLP) analysis of ribosomal (r) DNA was conducted on Uncinaria stenocephala, Ancylostoma caninum, A. tubaeforme and A. ceylanicum. The rDNA region spanning the first and second internal transcribed spacers (ITS1 and ITS2) plus the 5.8S (ITS+) gene was amplified by PCR from each of the species, digested separately with 9 restriction endonucleases and the fragments separated by agarose gel electrophoresis. Characteristic PCR-RFLP patterns were produced for each morphologically defined species using some of the endonucleases. The present study demonstrated that the ITS+ provides genetic markers for the delineation of each species examined and suggests that this region of rDNA will be useful for the identification of other hookworms from a range of hosts. The results are likely to have important implications for studying the genetic structure of hookworm populations, the systematics and the epidemiology of hookworm infections.

References

Dec 1, 1992·Molecular Phylogenetics and Evolution·D M WessonF H Collins
Jan 25, 1992·Nucleic Acids Research·C Schlötterer, D Tautz
Aug 1, 1990·Current Problems in Pediatrics·L K ZeltzerN L Schechter
Oct 25, 1990·Nucleic Acids Research·J M Hancock, G A Dover
Dec 1, 1988·Australian and New Zealand Journal of Medicine·T J Croese
Jan 1, 1986·Cold Spring Harbor Symposia on Quantitative Biology·K MullisH Erlich
Mar 1, 1995·International Journal for Parasitology·A J CampbellN B Chilton
Feb 1, 1993·Molecular and Biochemical Parasitology·J Bowles, D P McManus
Feb 1, 1993·International Journal for Parasitology·I BeveridgeR H Andrews
May 25, 1993·Nucleic Acids Research·R B GasserI Beveridge
Oct 1, 1995·Molecular and Cellular Probes·R B Gasser, H Hoste
Jan 1, 1961·Journal of Helminthology·S YAMAGUTI

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Citations

Mar 8, 2012·The Journal of Parasitology·Janice L LiottaDwight D Bowman
Mar 8, 2012·The Journal of Parasitology·Janice L LiottaDwight D Bowman
Mar 8, 2012·The Journal of Parasitology·Janice L LiottaDwight D Bowman
Mar 8, 2012·The Journal of Parasitology·Janice L LiottaDwight D Bowman
Aug 3, 2010·Transactions of the Royal Society of Tropical Medicine and Hygiene·Megumi SatoJitra Waikagul
Jul 10, 1998·Molecular and Biochemical Parasitology·R B GasserN B Chilton
May 23, 2006·Veterinary Parasitology·Leandra M Clara e SilvaElida M L Rabelo
Feb 18, 2014·International Journal for Parasitology. Parasites and Wildlife·Felicity A SmoutLee F Skerratt
May 24, 2006·Molecular and Cellular Probes·Johanna M de GruijterRobin B Gasser
Oct 21, 2016·International Journal for Parasitology. Parasites and Wildlife·Julia Angélica Gonçalves SilveiraRoselene Ecco
Dec 23, 2016·PLoS Neglected Tropical Diseases·Kenneth R HawkinsTala de Los Santos
Apr 15, 2014·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Marcello Otake OtakeJitra Waikagul
Jan 8, 2011·The American Journal of Tropical Medicine and Hygiene·Megumi SatoJitra Waikagul
Mar 22, 2007·The Korean Journal of Parasitology·Tai-Soon YongHan-Jong Rim
Aug 3, 2016·The American Journal of Tropical Medicine and Hygiene·Elise M O'Connell, Thomas B Nutman
Dec 23, 2008·Expert Review of Molecular Diagnostics·Robin B GasserBronwyn E Campbell
Feb 22, 2017·Journal of Helminthology·X L ShiG Q Li
Apr 3, 2018·Journal of Helminthology·A Merino-TejedorAnnette Olsen
Apr 2, 2020·Parasites & Vectors·Mureille Carole Tchami MbagniaFlobert Njiokou
Nov 25, 2020·Animals : an Open Access Journal From MDPI·Doolyawat KladkempetchSaruda Tiwananthagorn
Feb 19, 1998·International Journal for Parasitology·R B Gasser
Mar 25, 2000·International Journal for Parasitology·R B Gasser, S E Newton
Aug 24, 1999·Veterinary Parasitology·R B Gasser

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