Genetic modification of mesenchymal stem cells to overexpress CXCR4 and CXCR7 does not improve the homing and therapeutic potentials of these cells in experimental acute kidney injury

Stem Cells and Development
Yousof GheisariSirous Zeinali

Abstract

The therapeutic potential of bone marrow mesenchymal stem cells (MSCs) in kidney failure has been examined in some studies. However, recent findings indicate that after transplantation, these cells home to kidneys at very low levels. Interaction of stromal derived factor-1 (SDF-1) with its receptor, CXCR4, is of pivotal importance in migration and homing. Recently, CXCR7 has also been recognized as another SDF-1 receptor that interacts with CXCR4 and modulates its functions. In this study, CXCR4 and CXCR7 were separately and simultaneously overexpressed in BALB/c bone marrow MSCs by using a lentiviral vector system and the homing and renoprotective potentials of these cells were evaluated in a mouse model of cisplatin-induced acute kidney injury. Using flow cytometry, immunohistochemistry, and real-time PCR methods for detection of GFP-labeled MSCs, we found that although considerably entrapped in lungs, native MSCs home very rarely to kidneys and bone marrow and this rate cannot be significantly affected by CXCR4 and/or CXCR7 upregulation. Transplantation of neither native nor genetically engineered MSCs ameliorated kidney failure. We concluded that overexpression of CXCR4 and CXCR7 receptors in murine MSCs cannot improve the ...Continue Reading

References

Jul 17, 1981·European Journal of Pharmacology·S Castellani, P M Adams
Dec 6, 2000·The Journal of Clinical Investigation·T PonomaryovT Lapidot
Jun 15, 2001·Kidney International·P K ChatterjeeC Thiemermann
Apr 23, 2003·Journal of the American Society of Nephrology : JASN·Fangming LinPeter Igarashi
Jul 17, 2003·The Journal of Clinical Investigation·Orit KolletTsvee Lapidot
Aug 5, 2003·Journal of Cellular Biochemistry·Melody BaddooDonald G Phinney
Nov 18, 2003·British Journal of Haematology·Lindolfo da Silva Meirelles, Nance Beyer Nardi
Jun 24, 2004·Journal of the American Society of Nephrology : JASN·Marina MorigiGiuseppe Remuzzi
Jan 25, 2005·Lancet·A Meguid El Nahas, Aminu K Bello
Feb 17, 2005·American Journal of Physiology. Renal Physiology·Florian TögelChristof Westenfelder
Mar 9, 2005·Kidney International. Supplement·Philip Kam-Tao LiUNKNOWN Participants of ISN Consensus Workshop on Prevention of Progression of Renal Disease
Apr 21, 2005·Kidney International·Florian TögelChristof Westenfelder
Jul 12, 2005·The Journal of Clinical Investigation·Diane Krause, Lloyd G Cantley
Jun 23, 2006·Journal of the American Society of Nephrology : JASN·Uta KunterJürgen Floege
Nov 17, 2006·Cancer Research·Yi Fu ZhouGregg L Semenza
Jul 28, 2007·Journal of the American Society of Nephrology : JASN·Baoyuan BiLloyd G Cantley
Sep 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Frederic SierroFabienne Mackay
Nov 6, 2007·Journal of the American Society of Nephrology : JASN·Elena LazzeriPaola Romagnani
Feb 13, 2008·The Journal of Experimental Medicine·Benedetta MazzinghiPaola Romagnani
Jun 21, 2008·Stem Cells and Development·Florian TögelChristof Westenfelder
Oct 11, 2008·Nature Reviews. Drug Discovery·Norberto PericoGiuseppe Remuzzi
Dec 20, 2008·Stem Cells·Cristianne Silva AlexandreLúcia Andrade
Feb 19, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Ingrid StrooJaklien C Leemans
Apr 25, 2009·Journal of the American Society of Nephrology : JASN·Stefania BrunoGiovanni Camussi

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Citations

Jan 10, 2013·In Vitro Cellular & Developmental Biology. Animal·Abdollah Mohammadi-SangcheshmehMasoud Soleimani
Jun 19, 2013·Stem Cells and Development·Martin J HoogduijnCarla C Baan
May 28, 2013·Molekuliarnaia biologiia·S Sh KarshievaA V Beliavskiĭ
Nov 13, 2013·Nature Reviews. Rheumatology·Alan Tyndall
Sep 1, 2014·Cell and Tissue Research·Abbas HajifathaliMahsa Rasekhi
Jul 19, 2015·Immunology Letters·Anne S CornelissenCarlijn Voermans
Jun 25, 2013·Journal of Cellular Biochemistry·Nanmei LiuJinyuan Zhang
Sep 19, 2014·Cell Biology International·Hojjat Naderi-MeshkinNaghmeh Ahmadiankia
Nov 5, 2013·Biochemical and Biophysical Research Communications·Qiang LiPeisheng Jin
Jan 2, 2014·BioMed Research International·Leah A Marquez-Curtis, Anna Janowska-Wieczorek
Oct 5, 2013·Journal of the American Society of Nephrology : JASN·Daisuke KatagiriEisei Noiri
Nov 30, 2019·Frontiers in Endocrinology·Babak ArjmandBagher Larijani
Sep 29, 2019·Journal of Cellular and Molecular Medicine·Leyla Fath-BayatiEhsan Sharif-Paghaleh
Oct 14, 2020·Stem Cells and Development·Zhihua RenRiyi Shi
May 24, 2019·IScience·Mujib UllahAvnesh S Thakor
Jun 28, 2021·Cellular & Molecular Biology Letters·Shabnam BabazadehMohamad Zamani-Ahmadmahmudi

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Methods Mentioned

BETA
flow cytometry
PCR
electrophoresis
flow

Software Mentioned

SPSS
CyFlow
FloMax

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