Genetic modification of respiratory capacity in potato

Plant Physiology
C HiserL McIntosh

Abstract

Mitochondrial respiration was altered in transgenic potato (Solanum tuberosum) lines by overexpression of the alternative oxidase Aox1 gene. Overexpressing lines showed higher levels of Aox1 mRNA, increased levels of alternative oxidase protein(s), and an unusual higher molecular weight polypeptide, which may be a normal processing/modification intermediate. Evidence suggests that the alternative oxidase protein is further processed/modified beyond removal of the transit peptide. Addition of pyruvate to mitochondria oxidizing succinate or NADH increased the alternative pathway capacity but did not eliminate the difference in the capacity between these two substrates. Induction of alternative pathway capacity by aging of tubers appeared to be more dependent on increased levels of alternative oxidase protein than changes in its oxidation state. In leaf and tuber mitochondria, overexpressing lines possessed higher alternative pathway capacity than the control line, which suggests that changing the alternative oxidase protein level by genetic engineering can effectively change alternative pathway capacity.

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A M Kumar, D Söll
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D M Rhoads, L McIntosh
Aug 23, 1991·Biochimica Et Biophysica Acta·A L Moore, J N Siedow
Mar 25, 1989·Nucleic Acids Research·T C VerwoerdA Hoekema
Jun 1, 1986·Analytical Biochemistry·E LarsonA Jagendorf
Apr 1, 1995·Plant Physiology·J WhelanD A Day
Dec 1, 1993·Plant Physiology·J WhelanD A Day

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Citations

Jan 12, 2000·Pediatric Clinics of North America·J D McAllister, K A Gnauck
Aug 28, 2012·Nature Biotechnology·Damar Lizbeth López-Arredondo, Luis Herrera-Estrella
Aug 30, 2005·Plant Physiology·Miquel Ribas-CarboJaume Flexas
Dec 7, 2002·The Plant Cell·Olga V KarpovaKathleen J Newton
Mar 5, 2011·Annual Review of Plant Biology·Tzyy-Jen Chiou, Shu-I Lin
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Greg C. Vanlerberghe, Lee McIntosh
Oct 28, 2006·Annual Review of Plant Biology·Daniel P Schachtman, Ryoung Shin
Jan 9, 1999·The Journal of Biological Chemistry·M TanudjiJ Whelan
Jul 13, 2002·Plant Physiology·Michael James ConsidineA Harvey Millar

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