Genetic modulation in Be-78 and Y Trypanosoma cruzi strains after long-term infection in Beagle dogs revealed by molecular markers

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
Vanja Maria VelosoMaria Terezinha Bahia

Abstract

The genetic profile of Trypanosoma cruzi was evaluated in parasite populations isolated from Beagle dogs experimentally infected with Be-78 and Y strains that present distinct biological and genetic characteristics. Molecular characterization of the isolates obtained 30days and 2years after infection was carried out. For typing MLEE, sequence polymorphisms of the mitochondrial cytochrome oxidase subunit II gene (COII) and RAPD profiles were used. The profiles of MLEE were the same for the parental Be-78 strains as their respective isolates. However, changes of MLEE profile were observed in two T. cruzi isolates from dogs inoculated with Y strain. Changes in the mitochondrial DNA (COII) and RAPD profiles of the Y strain were also observed. The dendogram constructed by UPGMA with RAPD results indicated two major branches. Global data show that the genetic modulation in polyclonal strains during the long-term infection occurred and was strain-dependent. This study still suggests that each host (here each dog) harbors a determinate T. cruzi population that may change or be modulated throughout long-term infection. This might to hinder the observation of correlation between the genetics of T. cruzi and their biological properties an...Continue Reading

References

Jan 1, 1979·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A J RomanhaV Kilgour
Jan 1, 1992·Memórias do Instituto Oswaldo Cruz·M de LanaW L Tafuri
Jan 1, 1989·Revista Da Sociedade Brasileira De Medicina Tropical·E ChiariC A Chiari
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M TibayrencF J Ayala
Sep 1, 1994·Computer Applications in the Biosciences : CABIOS·Y Van de Peer, R De Wachter
Jul 1, 1993·Molecular and Biochemical Parasitology·M SteindelA J Simpson
Dec 22, 1999·Microbes and Infection·M Tibayrenc, F J Ayala
Nov 23, 2000·Molecular and Biochemical Parasitology·S BrisseM Tibayrenc
Oct 31, 2001·Memórias do Instituto Oswaldo Cruz·V M VelosoM T Bahia
Aug 14, 2002·Experimental Parasitology·M J de O ToledoW L Tafuri
Oct 22, 2002·Acta Tropica·Paulo Marcos da Matta GuedesMaria Terezinha Bahia
Dec 25, 2002·Antimicrobial Agents and Chemotherapy·Max Jean de Ornelas ToledoMarta de Lana
Jun 1, 1987·Parasitology Today·M Tibayrenc, F J Ayala
Apr 13, 2006·PLoS Pathogens·Jorge M de FreitasSérgio D J Pena
Oct 1, 2008·Experimental Parasitology·Fabiane Matos Dos SantosMaria Terezinha Bahia
Dec 23, 2009·Memórias do Instituto Oswaldo Cruz·B ZingalesUNKNOWN Second Satellite Meeting

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Mar 8, 2018·Arquivos Brasileiros De Cirurgia Digestiva : ABCD = Brazilian Archives of Digestive Surgery·Carlos Edmundo Rodrigues FontesAretuza Zaupa Gasparim

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