Genetic organization of the catabolic plasmid pJP4 from Ralstonia eutropha JMP134 (pJP4) reveals mechanisms of adaptation to chloroaromatic pollutants and evolution of specialized chloroaromatic degradation pathways

Environmental Microbiology
N TrefaultB González

Abstract

Ralstonia eutropha JMP134 (pJP4) is a useful model for the study of bacterial degradation of substituted aromatic pollutants. Several key degrading capabilities, encoded by tfd genes, are located in the 88 kb, self-transmissible, IncP-1 beta plasmid pJP4. The complete sequence of the 87,688 nucleotides of pJP4, encoding 83 open reading frames (ORFs), is reported. Most of the coding sequence corresponds to a well-conserved IncP-1 beta backbone and the previously reported tfd genes. In addition, we found hypothetical proteins putatively involved in the transport of aromatic compounds and short-chain fatty acid oxidation. ORFs related to mobile elements, including the Tn501-encoded mercury resistance determinants, an IS1071-based composite transposon and a cryptic class II transposon, are also present in pJP4. These mobile elements are inefficient in transposition and are located in two regions of pJP4 that are rich in remnants of lateral gene transfer events. pJP4 plasmid was able to capture chromosomal genes and form hybrid plasmids with the IncP-1 alpha plasmid RP4. These observations are integrated into a model for the evolution of pJP4, which reveals mechanisms of bacterial adaptation to degrade pollutants.

References

Dec 1, 1990·Journal of Bacteriology·R S BurlageF Larimer
Aug 1, 1987·Molecular & General Genetics : MGG·M MergeayL Fabry
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D GhosalA M Chakrabarty
Mar 1, 1981·Journal of Bacteriology·C I Kado, S T Liu
Dec 1, 1994·Biodegradation·R C WyndhamM C Peel
Aug 1, 1994·Journal of Bacteriology·M I Ramos-GonzálezJ L Ramos
May 14, 1999·Trends in Biotechnology·K N Timmis, D H Pieper
Feb 2, 2002·Applied and Environmental Microbiology·Andrew C Hawkins, Caroline S Harwood
Jul 11, 2002·Journal of Bacteriology·Iris PlumeierDietmar H Pieper
Oct 26, 2002·Journal of Bacteriology·Johannes GescherGeorg Fuchs
Jan 22, 2003·Environmental Microbiology·Alicia GreatedChristopher M Thomas

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Citations

Feb 3, 2005·Archives of Microbiology·Monika ThielMichael Schlömann
Dec 23, 2004·Applied Microbiology and Biotechnology·Dietmar H Pieper
Sep 5, 2009·Applied Microbiology and Biotechnology·Bin CaoKai-Chee Loh
Jun 5, 2013·Ecotoxicology and Environmental Safety·Surasak Siripornadulsil, Wilailak Siripornadulsil
Mar 10, 2009·Applied and Environmental Microbiology·Peter F AndeerStuart E Strand
Jan 5, 2006·Applied and Environmental Microbiology·Masahiro SotaMasataka Tsuda
Sep 19, 2006·Journal of Bacteriology·Juanita Larraín-LintonBernardo González
Dec 19, 2006·Journal of Bacteriology·Masaki ShintaniHideaki Nojiri
Jan 10, 2012·Journal of Bacteriology·Hirokazu YanoEva M Top
Feb 18, 2006·Journal of Bacteriology·Muriel GaillardJan Roelof van der Meer
Mar 5, 2014·Canadian Journal of Microbiology·Surasak SiripornadulsilWilailak Siripornadulsil
Jan 11, 2012·Bioscience, Biotechnology, and Biochemistry·Hideaki Nojiri
Nov 17, 2006·Environmental Microbiology·Michael Y Galperin
Jun 18, 2005·Current Opinion in Biotechnology·Jonathan J Dennis

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