Genetic organization of the Pantoea stewartii subsp. stewartii hrp gene cluster and sequence analysis of the hrpA, hrpC, hrpN, and wtsE operons

Molecular Plant-microbe Interactions : MPMI
R D FrederickDavid L Coplin

Abstract

The hrp/wts gene cluster of Pantoea stewartii subsp. stewartii is required for pathogenicity on sweet corn and the ability to elicit a hypersensitive response (HR) in tobacco. Site-directed transposon mutagenesis and nucleotide sequencing were used to identify hrp/wts genes within the left 20 kb of this cluster. Seventeen open reading frames (ORFs) comprise seven genetic complementation groups. These ORFs share homology with hrp and dsp genes from Erwinia amylovora, Erwinia chrysanthemi, and Pseudomonas syringae pathovars and have been designated, in map order, wtsF, wtsE, hrpN, hrpV, hrpT, hrcC, hrpG, hrpF, hrpE, hrpD, hrcJ, hrpB, hrpA, hrpS, hrpY, hrpX, and hrpL. Putative hrp consensus promoter sequences were identified upstream of hrpA, hrpF, hrpN, and wtsE. Expression of the hrpA, hrpC, and wtsE operons was regulated by HrpS. Transposon mutations in all of the hrp operons abolished pathogenicity and HR elicitation, except for the hrpN and hrpV mutants, which were still pathogenic. hrpS, hrpXY, and hrpL regulatory mutations abolished HrpN synthesis, whereas secretory mutations in the hrpC, hrpA, and hrpJ operons permitted intracellular HrpN synthesis. wtsEF mutants were not pathogenic but still produced HrpN and elicited the...Continue Reading

References

Nov 1, 1986·Journal of Bacteriology·D L CoplinE S Haas
May 14, 1969·Journal of Molecular Biology·H W Boyer, D Roulland-Dussoix
Oct 1, 1981·Applied and Environmental Microbiology·D L CoplinR E Whitmoyer
Apr 1, 1996·Molecular Microbiology·P WattiauG R Cornelis
May 1, 1996·Molecular Microbiology·A J BogdanoveF Van Gijsegem
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·E RoineS Y He
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Mar 14, 1998·Proceedings of the National Academy of Sciences of the United States of America·A J BogdanoveS V Beer
Aug 29, 1998·Journal of Bacteriology·G PrestonA Collmer
Dec 6, 2000·Molecular Plant-microbe Interactions : MPMI·Z ChenB N Kunkel
Jan 3, 2001·Proceedings of the National Academy of Sciences of the United States of America·J LeeT Nürnberger
Mar 30, 2001·Molecular Plant-microbe Interactions : MPMI·H MorI Barash
Oct 19, 2001·Molecular Plant-microbe Interactions : MPMI·M AhmadD L Coplin
Jan 1, 1997·Annual Review of Phytopathology·P B Lindgren

❮ Previous
Next ❯

Citations

Oct 24, 2003·Current Opinion in Microbiology·Mark J PallenIan R Henderson
Dec 16, 2005·Molecular Plant-microbe Interactions : MPMI·William NasserMartine Boccara
Nov 1, 2006·Molecular Plant-microbe Interactions : MPMI·Xiaoyan TangJian-Min Zhou
May 18, 2007·Molecular Plant-microbe Interactions : MPMI·Ernst WeberRalf Koebnik
Sep 13, 2008·Molecular Plant-microbe Interactions : MPMI·Carmen M HerreraSusanne B von Bodman
May 19, 2009·Molecular Plant-microbe Interactions : MPMI·Jong Hyun HamDavid L Coplin
Oct 19, 2001·Molecular Plant-microbe Interactions : MPMI·M AhmadD L Coplin
Jul 10, 2012·Applied and Environmental Microbiology·Valdir R CorreaMargaret G Redinbaugh
Dec 26, 2006·Applied and Environmental Microbiology·Shihui YangChing-Hong Yang
Nov 15, 2011·Journal of Bacteriology·John StavrinidesDavid S Guttman
Nov 18, 2005·Journal of Bacteriology·Youfu ZhaoGeorge W Sundin
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Anja Brencic, Stephen C Winans
May 6, 2003·Annual Review of Phytopathology·Susanne B Von BodmanDavid L Coplin
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sruti DebRoySheng Yang He
Sep 1, 2003·Molecular Plant Pathology·Dawn L ArnoldRobert W Jackson
Jun 26, 2015·FEMS Microbiology Reviews·Alyssa M Walterson, John Stavrinides
May 20, 2015·Molecular Genetics and Genomics : MGG·Morgan W B KirzingerJohn Stavrinides
Apr 6, 2005·FEMS Microbiology Reviews·Mark J PallenChristopher M Bailey
Jan 22, 2011·FEMS Microbiology Reviews·Geetanchaly Nadarasah, John Stavrinides
Feb 21, 2013·FEMS Microbiology Letters·Sabrina SiamerMarie-Anne Barny

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.