Abstract
The hrp/wts gene cluster of Pantoea stewartii subsp. stewartii is required for pathogenicity on sweet corn and the ability to elicit a hypersensitive response (HR) in tobacco. Site-directed transposon mutagenesis and nucleotide sequencing were used to identify hrp/wts genes within the left 20 kb of this cluster. Seventeen open reading frames (ORFs) comprise seven genetic complementation groups. These ORFs share homology with hrp and dsp genes from Erwinia amylovora, Erwinia chrysanthemi, and Pseudomonas syringae pathovars and have been designated, in map order, wtsF, wtsE, hrpN, hrpV, hrpT, hrcC, hrpG, hrpF, hrpE, hrpD, hrcJ, hrpB, hrpA, hrpS, hrpY, hrpX, and hrpL. Putative hrp consensus promoter sequences were identified upstream of hrpA, hrpF, hrpN, and wtsE. Expression of the hrpA, hrpC, and wtsE operons was regulated by HrpS. Transposon mutations in all of the hrp operons abolished pathogenicity and HR elicitation, except for the hrpN and hrpV mutants, which were still pathogenic. hrpS, hrpXY, and hrpL regulatory mutations abolished HrpN synthesis, whereas secretory mutations in the hrpC, hrpA, and hrpJ operons permitted intracellular HrpN synthesis. wtsEF mutants were not pathogenic but still produced HrpN and elicited the...Continue Reading
References
May 20, 1992·Journal of Molecular Biology·G von Heijne
Jul 3, 1992·Science·Z M WeiS V Beer
Aug 1, 1991·Journal of Bacteriology·T MichielsG R Cornelis
Feb 1, 1988·Journal of Bacteriology·P J DolphD L Coplin
Nov 1, 1986·Journal of Bacteriology·D L CoplinE S Haas
May 14, 1969·Journal of Molecular Biology·H W Boyer, D Roulland-Dussoix
Oct 1, 1981·Applied and Environmental Microbiology·D L CoplinR E Whitmoyer
Sep 1, 1995·Molecular Plant-microbe Interactions : MPMI·G PrestonA Collmer
Sep 1, 1995·Molecular Plant-microbe Interactions : MPMI·H C HuangW L Deng
Nov 1, 1995·Journal of Bacteriology·Z M Wei, S V Beer
Jan 1, 1995·Molecular Plant-microbe Interactions : MPMI·J M Lorang, N T Keen
Sep 1, 1994·Molecular Plant-microbe Interactions : MPMI·D W BauerA Collmer
Jul 29, 1994·Science·M Russel
Aug 1, 1993·Molecular Microbiology·R D FrederickD L Coplin
Jul 1, 1995·Molecular Plant-microbe Interactions : MPMI·D W BauerA Collmer
Mar 1, 1996·Journal of Bacteriology·A J BogdanoveS V Beer
Apr 1, 1996·Molecular Microbiology·P WattiauG R Cornelis
May 1, 1996·Molecular Microbiology·A J BogdanoveF Van Gijsegem
Feb 1, 1996·Molecular Microbiology·J R AlfanoA Collmer
Mar 1, 1997·Journal of Bacteriology·J F KimS V Beer
Jan 1, 1997·Protein Engineering·H NielsenG von Heijne
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·E RoineS Y He
Sep 1, 1995·Genome Research·K C WorleyR F Smith
May 1, 1997·Molecular Plant-microbe Interactions : MPMI·A MukherjeeA K Chatterjee
Jul 1, 1997·Molecular Plant-microbe Interactions : MPMI·J R AlfanoA Collmer
Jul 1, 1997·Molecular Plant-microbe Interactions : MPMI·R NizanS Manulis
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Sep 19, 1997·Journal of Bacteriology·J R Alfano, A Collmer
Dec 12, 1997·FEBS Letters·E RoineM Romantschuk
Jan 13, 1998·Molecular Microbiology·S GaudriaultM A Barny
Mar 14, 1998·Proceedings of the National Academy of Sciences of the United States of America·A J BogdanoveS V Beer
Jun 5, 1998·Molecular Plant-microbe Interactions : MPMI·J F KimS V Beer
Aug 29, 1998·Journal of Bacteriology·W L DengH C Huang
Aug 29, 1998·Journal of Bacteriology·G PrestonA Collmer
Sep 28, 1998·Journal of Bacteriology·J F Kim, S V Beer
Mar 27, 1999·Journal of Bacteriology·W L Deng, H C Huang
Mar 30, 1999·Molecular Microbiology·D M Anderson, O Schneewind
May 21, 1999·Science·J E Galán, A Collmer
Jun 11, 1999·Molecular Microbiology·M B Mudgett, B J Staskawicz
Feb 19, 2000·Current Opinion in Microbiology·S KjemtrupJ L Dangl
May 3, 2000·Molecular Microbiology·F Van GijsegemC Boucher
Dec 6, 2000·Molecular Plant-microbe Interactions : MPMI·Z ChenB N Kunkel
Jan 3, 2001·Proceedings of the National Academy of Sciences of the United States of America·J LeeT Nürnberger
Feb 24, 2001·Molecular Plant-microbe Interactions : MPMI·W HuS Y He
Mar 30, 2001·Molecular Plant-microbe Interactions : MPMI·H MorI Barash
Oct 19, 2001·Molecular Plant-microbe Interactions : MPMI·M AhmadD L Coplin
Jan 1, 1997·Annual Review of Phytopathology·P B Lindgren
Citations
Nov 7, 2006·Applied Microbiology and Biotechnology·Qiong WuLian-Hui Zhang
Sep 2, 2005·Planta·Young-Sun JangMyeong-Hyeon Wang
Oct 24, 2003·Current Opinion in Microbiology·Mark J PallenIan R Henderson
Sep 1, 2002·Molecular Plant Pathology·Asita ChatterjeeArun K Chatterjee
Dec 16, 2005·Molecular Plant-microbe Interactions : MPMI·William NasserMartine Boccara
Jan 13, 2006·Molecular Plant-microbe Interactions : MPMI·Tristan BoureauMarie-Anne Barny
Oct 7, 2006·Molecular Plant-microbe Interactions : MPMI·Jong Hyun HamDavid L Coplin
Nov 1, 2006·Molecular Plant-microbe Interactions : MPMI·Xiaoyan TangJian-Min Zhou
May 18, 2007·Molecular Plant-microbe Interactions : MPMI·Ernst WeberRalf Koebnik
Sep 13, 2008·Molecular Plant-microbe Interactions : MPMI·Carmen M HerreraSusanne B von Bodman
May 19, 2009·Molecular Plant-microbe Interactions : MPMI·Jong Hyun HamDavid L Coplin
Oct 19, 2001·Molecular Plant-microbe Interactions : MPMI·M AhmadD L Coplin
Sep 19, 2002·Molecular Plant-microbe Interactions : MPMI·Asita ChatterjeeArun K Chatterjee
Dec 4, 2003·Molecular Plant-microbe Interactions : MPMI·Asita ChatterjeeArun K Chatterjee
Mar 26, 2003·Molecular Plant-microbe Interactions : MPMI·Tarja KariolaE Tapio Palva
Mar 26, 2003·Molecular Plant-microbe Interactions : MPMI·Massimo MerighiDavid L Coplin
Feb 18, 2004·Molecular Plant-microbe Interactions : MPMI·Jong Hyun HamAlan Collmer
Jun 16, 2004·Molecular Plant-microbe Interactions : MPMI·Clemencia M RojasAlan Collmer
Sep 24, 2004·Molecular Plant-microbe Interactions : MPMI·Maria C HolevaIan K Toth
Oct 24, 2008·Phytopathology·Fengquan LiuHansong Dong
Jul 10, 2012·Applied and Environmental Microbiology·Valdir R CorreaMargaret G Redinbaugh
Dec 26, 2006·Applied and Environmental Microbiology·Shihui YangChing-Hong Yang
Jul 4, 2006·Journal of Bacteriology·Massimo MerighiDavid L Coplin
Nov 15, 2011·Journal of Bacteriology·John StavrinidesDavid S Guttman
Jul 21, 2004·Journal of Bacteriology·John Stavrinides, David S Guttman
Sep 3, 2004·Journal of Bacteriology·Amanda S EngledowCarlos F Gonzalez
Nov 18, 2005·Journal of Bacteriology·Youfu ZhaoGeorge W Sundin
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Anja Brencic, Stephen C Winans
May 6, 2003·Annual Review of Phytopathology·Susanne B Von BodmanDavid L Coplin
Jul 18, 2012·BMC Microbiology·Heath E O'BrienDavid S Guttman
Jun 8, 2012·BMC Microbiology·Maria Augusta SchmidtRose A Monteiro
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sruti DebRoySheng Yang He
Dec 1, 2011·FEBS Open Bio·Sabrina SiamerMarie-Anne Barny
Jun 28, 2005·Molecular Microbiology·Chia-Fong WeiHsiou-Chen Huang
Mar 1, 2005·Molecular Plant Pathology·Chang-Sik OhSteven V Beer
Aug 28, 2003·Molecular Microbiology·Jorge L BadelSheng Yang He
Sep 1, 2003·Molecular Plant Pathology·Dawn L ArnoldRobert W Jackson
Nov 21, 2008·Molecular Plant Pathology·Jong Hyun HamDavid Coplin
May 1, 2007·Molecular Plant Pathology·Chang-Sik OhSteven V Beer
Jul 6, 2011·Molecular Plant Pathology·M Caroline Roper
Nov 25, 2011·Molecular Microbiology·Xiaolei WangSusanne B von Bodman
Jun 30, 2010·Molecular Microbiology·Xuan YiChing-Hong Yang
Jun 26, 2015·FEMS Microbiology Reviews·Alyssa M Walterson, John Stavrinides
May 20, 2015·Molecular Genetics and Genomics : MGG·Morgan W B KirzingerJohn Stavrinides
Feb 3, 2015·Molecular Plant Pathology·Alexandre DegraveMarie-Anne Barny
Apr 6, 2005·FEMS Microbiology Reviews·Mark J PallenChristopher M Bailey
Jan 22, 2011·FEMS Microbiology Reviews·Geetanchaly Nadarasah, John Stavrinides
Mar 8, 2005·FEMS Microbiology Letters·Massimo MerighiDavid L Coplin
Feb 21, 2013·FEMS Microbiology Letters·Sabrina SiamerMarie-Anne Barny
May 19, 2016·PLoS Pathogens·Lin JinDavid Mackey