Feb 12, 1998

Genetic patterning of the developing mouse tail at the time of posterior neuropore closure

Developmental Dynamics : an Official Publication of the American Association of Anatomists
F GofflotGillian M Morriss-Kay

Abstract

Posterior neuropore (PNP) closure coincides with the end of gastrulation, marking the end of primary neurulation and primary body axis formation. Secondary neurulation and axis formation involve differentiation of the tail bud mesenchyme. Genetic control of the primary-secondary transition is not understood. We report a detailed analysis of gene expression in the caudal region of day 10 mouse embryos during primary neuropore closure. Embryos were collected at the 27-32 somite stage, fixed, processed for whole mount in situ hybridisation, and subsequently sectioned for a more detailed analysis. Genes selected for study include those involved in the key events of gastrulation and neurulation at earlier stages and more cranial levels. Patterns of expression within the tail bud, neural plate, recently closed neural tube, notochord, hindgut, mesoderm, and surface ectoderm are illustrated and described. Specifically, we report continuity of expression of the genes Wnt5a, Wnt5b, Evx1, Fgf8, RARgamma, Brachyury, and Hoxb1 from primitive streak and node into subpopulations of the tail bud and caudal axial structures. Within the caudal notochord, developing floorplate, and hindgut, HNF3alpha, HNF3beta, Shh, and Brachyury expression domai...Continue Reading

  • References19
  • Citations39

References

Mentioned in this Paper

Embryo
Hedgehog Proteins
Neural Tube
Primitive Streak
Hindgut
EVX1 gene
Hoxb1
Wnt5a
FGF8 protein, human
SHH protein, human

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