Genetic pleiotropy in Saccharomyces cerevisiae quantified by high-resolution phenotypic profiling

Molecular Genetics and Genomics : MGG
Elke EricsonAnders Blomberg

Abstract

Genetic pleiotropy, the ability of a mutation in a single gene to give rise to multiple phenotypic outcomes, constitutes an important but incompletely understood biological phenomenon. We used a high-resolution and high-precision phenotypic profiling approach to quantify the fitness contribution of genes on the five smallest yeast chromosomes during different forms of environmental stress, selected to probe a wide diversity of physiological features. We found that the extent of pleiotropy is much higher than previously claimed; 17% of the yeast genes were pleiotropic whereof one-fifth were hyper-pleiotropic. Pleiotropic genes preferentially participate in functions related to determination of protein fate, cell growth and morphogenesis, signal transduction and transcription. Contrary to what has earlier been proposed we did not find experimental evidence for slower evolutionary rate of pleiotropic genes/proteins. We also refute the existence of phenotypic islands along chromosomes but report on a remarkable loss both of pleiotropy and of phenotypic penetrance towards chromosomal ends. Thus, the here reported features of pleiotropy both have implications on our understanding of evolutionary processes as well as the mechanisms un...Continue Reading

References

Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S F Altschul, D J Lipman
Mar 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J N DavidsonC B Kern
Feb 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·J W ThatcherW J Dickinson
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·K A Gottlieb, L P Villarreal
Jun 29, 2001·Nature·A E Hirsh, H B Fraser
Dec 26, 2001·Nucleic Acids Research·H W MewesB Weil
May 7, 2002·Nature Genetics·Martin J LercherLaurence D Hurst
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Regina Preisig-MüllerJürgen Daut
Jul 26, 2002·Nature·Guri GiaeverMark Johnston
Jul 30, 2002·Journal of Biology·Paul T Spellman, Gerald M Rubin
Jan 4, 2003·Nature·Zhenglong GuWen-Hsiung Li
Feb 11, 2003·Nature Genetics·Csaba Pál, Laurence D Hurst
Jun 28, 2003·Methods : a Companion to Methods in Enzymology·Ravi S Kamath, Julie Ahringer
Dec 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jonas WarringerAnders Blomberg
Jun 24, 2004·Proceedings. Biological Sciences·Sarah P Otto
Aug 13, 2004·Proceedings. Biological Sciences·Daniel E L Promislow
Sep 17, 2004·Molecular Biology and Evolution·Aaron E HirshDennis P Wall
Apr 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Dennis P WallMarcus W Feldman

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Citations

Dec 16, 2011·Proceedings. Biological Sciences·Suzanne C MillsTapio Mappes
Aug 5, 2011·BMC Bioinformatics·Tahir MehmoodLars Snipen
Aug 17, 2006·BMC Evolutionary Biology·Jonas Warringer, Anders Blomberg
May 9, 2008·PLoS Computational Biology·Lihua ZouHui Ge
Jan 5, 2011·Genetics·Pablo Razeto-BarryRodrigo A Vásquez
Jan 4, 2008·The American Naturalist·Judith E MankHans Ellegren
Nov 26, 2010·Current Opinion in Biotechnology·Anders Blomberg
Jun 5, 2007·Evolution; International Journal of Organic Evolution·Tim F CooperMichael Travisano
Oct 26, 2011·Evolution; International Journal of Organic Evolution·Pablo Razeto-Barry, Karin Maldonado
Jan 26, 2011·Molecular Ecology·Francisco A CubillosGianni Liti
Aug 22, 2006·Biochimica Et Biophysica Acta·Maria L GasparSusan A Henry
Jan 22, 2017·The FEBS Journal·José Carlos Ponce-RojasSoledad Funes
Jun 10, 2009·The Journal of Cell Biology·Helen E BurstonElizabeth Conibear
Jun 21, 2019·ELife·Karole N D'OrazioRachel Green
May 18, 2013·FEMS Yeast Research·Karen A HechtJeffrey L Brodsky
Jan 15, 2016·Open Biology·Katherine E VestPaul A Cobine
Jan 7, 2014·Genetics·Alan J HerrBradley D Preston
Apr 18, 2013·Molecular Systems Biology·Nurit AvrahamNaama Barkai
Dec 18, 2016·Molecular Systems Biology·Malene L UrbanusAlexander W Ensminger
Sep 17, 2014·The Journal of Cell Biology·Fred D MastJohn D Aitchison
May 3, 2016·Journal of the Royal Statistical Society. Series C, Applied Statistics·Jonathan HeydariDarren J Wilkinson
Sep 15, 2016·BioTechniques·Itahisa Hernández HidalgoJens Tyedmers
Dec 1, 2017·The Journal of Cell Biology·Michal Eisenberg-BordMaria Bohnert
May 22, 2013·The Journal of Cell Biology·Lee Zeev PetersShay Ben-Aroya
Jul 11, 2019·The Journal of Cell Biology·Yury S BykovJohn A G Briggs
Sep 11, 2019·Current Protocols in Molecular Biology·Rajaraman Gopalakrishnan, Fred Winston
Sep 26, 2007·Molecular Biology and Evolution·Judith E MankHans Ellegren

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