Genetic polymorphism and its potential relation to environmental stress in five populations of the European flounder Platichthys flesus, along the French Atlantic coast

Marine Environmental Research
J MarchandJ Laroche

Abstract

In this study, new DNA markers were explored for the flounder Platichthys flesus. cDNA and genomic sequences of the genes encoding the glyceraldehyde-3-phosphate-deshydrogenase (GAPDH), the cytosolic creatine kinase (CK), the prostaglandin D synthase (PGDS) and the betaine homocysteine methyltransferase (BHMT) were characterized. The tumour suppressor p53 gene structure was already described. A PCR-SSCP (Single Strand Conformation Polymorphism) analysis was finally conducted to study the genetic polymorphism of different populations of flounders collected along the French Atlantic coast. Four highly contaminated French estuaries (Seine, Vilaine, Loire and Gironde) were sampled and compared to a reference estuary (Ster) to explore possible selective effect of the environment on specific allelic frequencies. Our results showed that two loci p53 and PGDS, could be potential markers of chemical stress: p53A allele frequency increased in contaminated systems compared to the reference system. In the Vilaine estuary, PGDS polymorphism could be related to pesticide stress.

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Citations

Sep 5, 2012·Environmental Science and Pollution Research International·Estérine EvrardJean Laroche
Apr 10, 2019·Proceedings. Biological Sciences·Tuul SeppMathieu Giraudeau
Jun 22, 2018·Evolutionary Applications·Marion VittecoqFrédéric Thomas
Feb 2, 2021·Evolutionary Applications·S Elizabeth AlterEmmanuel Megafu

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