Genetic polymorphisms in tumour necrosis factor receptors (TNFRSF1A/1B ) illustrate differential treatment response to TNFα inhibitors in patients with Crohn's disease

BMJ Open Gastroenterology
Ahmad QasemSaleh A Naser

Abstract

Monoclonal antibodies inhibiting tumour necrosis factor-α (TNFα) signalling pathway (anti-TNFα) have been widely used in Crohn's disease (CD). However, treatment response varies among patients with CD and the clinical outcome is dependent on single nucleotide polymorphisms (SNP) in TNFα receptor superfamily 1A and 1B (TNFRSF1A/1B). We tested nine SNPs in TNFα, TNFRSF1A and TNFRSF1B by TaqMan genotyping from peripheral blood samples of 104 subjects. Additionally, we quantified the effects of these SNPs on their corresponding gene expression by RT-PCR and susceptibility to Mycobacterium avium subsp paratuberculosis (MAP) infection by IS900 nested PCR. Four SNPs (TNFα:rs1800629, TNFRSF1A:rs767455, TNFRSF1B:rs1061624 and TNFRSF1B:rs3397) were over-represented significantly (p<0.05) among patients with CD compared with healthy controls. The TNFRSF1A:rs767455 GG genotype was found in 15/54 patients with CD (28%), while it was only found in 2/50 healthy controls (4%) (OR 9.2, 95% CI 1.98 to 42.83). The TNFRSF1B:rs3397 TT genotype was found in 15/54 patients with CD (28%) compared with (4/50) healthy controls (8%) (OR 4.4, 95% CI 1.36 to 14.14). Furthermore, the SNPs TNFRSF1A:rs767455 and TNFRSF1B:rs3397 were associated with downregula...Continue Reading

References

Nov 30, 1999·Journal of Leukocyte Biology·C FratazziH G Remold
Oct 21, 2000·Gastroenterology·K A Papadakis, S R Targan
Jun 8, 2001·Mayo Clinic Proceedings·M BaghaiE L Matteson
Nov 27, 2001·Inflammatory Bowel Diseases·M A Ritz, R Jost
Dec 6, 2001·Lancet·H MarotteP Miossec
Sep 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tania Botha, Bernhard Ryffel
Apr 2, 2005·Inflammatory Bowel Diseases·António Carlos FerreiraFernando Tavarela-Veloso
Apr 22, 2005·The American Journal of Gastroenterology·Kevin A WaschkeGary E Wild
May 3, 2007·Annals of the Rheumatic Diseases·Xiaoquan ZhuZe Yang
Dec 25, 2007·Pharmacology & Therapeutics·Daniel TraceyPaul P Tak
Jan 30, 2010·Gastroenterology Research and Practice·Gisela ChelimskyThomas Chelimsky
Mar 16, 2012·Inflammatory Bowel Diseases·James CookeMiles Parkes
Apr 30, 2013·Neurology·Manuel ComabellaXavier Montalban
Jan 8, 2016·Clinical and Translational Gastroenterology·Giulia RodaJean-Frederic Colombel
Apr 12, 2018·Canadian Journal of Physiology and Pharmacology·Amna NaserSaleh A Naser

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Citations

Aug 11, 2020·Journal of Pediatric Gastroenterology and Nutrition·Sara Salvador-MartínLuis A López-Fernández
Jun 2, 2020·British Journal of Clinical Pharmacology·Sara Salvador-MartínLuis Andrés López-Fernández
Aug 23, 2020·International Journal of Molecular Sciences·Dania AlQasrawiSaleh A Naser
Jun 25, 2020·International Journal of Colorectal Disease·Lijun XuQing Zheng
Apr 4, 2021·Journal of Clinical Medicine·Grzegorz SobieszekTeresa Małecka-Massalska
May 13, 2021·Scientific Reports·Laura Sanchis-ArteroXavier Cortés-Rizo

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Methods Mentioned

BETA
genotyping
PCR
Pharmacotherapy
Assay

Software Mentioned

GraphPad Prism

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