Genetic profiles of transcriptomic clusters of childhood asthma determine specific severe subtype

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
Y-L YehY L Lee

Abstract

Previous studies have defined transcriptomic subtypes of adult asthma using samples of induced sputum and bronchial epithelium; however, those procedures are not readily applicable in the clinic, especially for childhood asthma. We aim to dissect the transcriptomic clusters of childhood asthma using highly variably expressed genes of peripheral blood mononuclear cells (PBMC) among patients. Gene expression of PBMC from 133 asthmatic children and 11 healthy controls was measured with Illumina microarrays. We applied the k-means clustering algorithm of 2048 genes to assign asthmatic children into clusters. Genes with differential expression between asthma clusters and healthy controls were used to investigate whether they could identify severe asthma of children and adults. We identified 3 asthma clusters with distinct inflammatory profiles in peripheral blood. Cluster 1 had the highest eosinophil count. Cluster 2 showed lower counts of both eosinophils and neutrophils. Cluster 3 had the highest neutrophil count and the poorest treatment control. Compared with other patients, Cluster 3 exhibited a unique gene expression pattern which was associated with changes in the glucocorticoid signalling and activation of the T helper 1/T h...Continue Reading

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Citations

Feb 23, 2019·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Alberta G A PaulJudith A Woodfolk
Nov 24, 2019·Allergy·Gopal Krishna R DhondalaySandra Andorf
Mar 7, 2020·Allergy, Asthma & Immunology Research·Heung Woo Park, Scott T Weiss
Jan 10, 2020·Physiological Reviews·Anuradha RaySally E Wenzel
Apr 25, 2020·International Journal of Molecular Sciences·Javier Perez-GarciaMaria Pino-Yanes
Feb 26, 2019·Frontiers in Pediatrics·Natalia Hernandez-PachecoCarlos Flores
Feb 24, 2021·Expert Review of Respiratory Medicine·Sara FontanellaAdnan Custovic
Apr 13, 2021·Biochemical Society Transactions·Emma de Jong, Anthony Bosco

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