PMID: 9642195Jun 27, 1998Paper

Genetic recombination in Bacillus subtilis 168: effects of recU and recS mutations on DNA repair and homologous recombination

Journal of Bacteriology
S FernándezJ C Alonso

Abstract

Bacillus subtilis recombination-deficient mutants were constructed by inserting a selectable marker (cat gene) into the yppB and ypbC coding regions. The yppB:cat and ypbC:cat null alleles rendered cells sensitive to DNA-damaging agents, impaired plasmid transformation (25- and 100-fold), and moderately affected chromosomal transformation when present in an otherwise Rec+ B. subtilis strain. The yppB gene complemented the defect of the recG40 strain. yppB and ypbC and their respective null alleles were termed "recU" and "recU1" (recU:cat) and "recS" and "recS1" (recS:cat), respectively. The recU and recS mutations were introduced into rec-deficient strains representative of the alpha (recF), beta (addA5 addB72), gamma (recH342), and epsilon (recG40) epistatic groups. The recU mutation did not modify the sensitivity of recH cells to DNA-damaging agents, but it did affect inter- and intramolecular recombination in recH cells. The recS mutation did not modify the sensitivity of addAB cells to DNA-damaging agents, and it marginally affected recF, recH, and recU cells. The recS mutation markedly reduced (about 250-fold) intermolecular recombination in recH cells, and there were reductions of 10- to 20-fold in recF, addAB, and recU c...Continue Reading

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Citations

Feb 24, 2001·Journal of Bacteriology·S A SciochettiG W Blakely
Dec 31, 2005·Journal of Bacteriology·Humberto SanchezJuan C Alonso
Dec 13, 2006·Molecular and Cellular Biology·Sudha SharmaPerry J Blackshear
Apr 27, 2011·FEMS Microbiology Reviews·Silvia AyoraJuan C Alonso
May 24, 2015·Nucleic Acids Research·Begoña CarrascoJuan C Alonso
Aug 15, 2020·Frontiers in Molecular Biosciences·María Moreno-Del AlamoJuan Carlos Alonso
Oct 11, 2012·Critical Reviews in Biochemistry and Molecular Biology·Dawit KidaneJuan C Alonso
Sep 24, 2014·Journal of Bacteriology·Wei QinXu Guang Xi
Dec 17, 2002·Oncogene·Hiroaki Nakayama
Jan 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·Silvia AyoraJuan C Alonso
Dec 5, 2008·Microbiology and Molecular Biology Reviews : MMBR·Mark S Dillingham, Stephen C Kowalczykowski
Aug 31, 2012·Microbiology and Molecular Biology Reviews : MMBR·Justin S LenhartLyle A Simmons
Aug 8, 2008·Nucleic Acids Research·Cristina CañasJuan C Alonso
Sep 19, 2002·Protein Science : a Publication of the Protein Society·Daniel J RigdenMark J Jedrzejas
May 13, 2009·Nucleic Acids Research·Paula P CardenasJuan C Alonso
Aug 29, 2000·Research in Microbiology·S FernándezJ C Alonso
May 9, 2009·Journal of Molecular Biology·Begoña CarrascoSilvia Ayora
May 24, 2011·Journal of Molecular Biology·Cristina CañasSilvia Ayora

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