Jan 1, 1980

Genetic recombination of herpes simplex virus, the role of the host cell and UV-irradiation of the virus

Molecular & General Genetics : MGG
U B DasGupta, W C Summers

Abstract

Recombination frequencies for two sets of genetic markers of herpes simplex virus were determined in various host cells with and without ultraviolet irradiation of the virus. UV irradiation increased the recombination frequency in all the cell types studied in direct proportion to the unrepaired lethal damage. In human skin fibroblasts derived from a patient with xeroderma pigmentosum (XP) of complementation group A, a given dose of UV stimulated recombination more than that in fibroblasts from normal individuals. On the other hand, UV stimulation of HSV recombination was slightly less than normal in fibroblasts derived from a patient with a variant form XP and from an ataxia telangiectasia patient. Caffeine, an agent known to inhibit repair of UV damage, reduced recombination in most of the cell types studied and did not suppress the UV-induced increase in recombination. These findings suggest that for virus DNA with the same number of unrepaired UV-lesions, each of the tested cell types promoted HSV-recombination to an equivalent extent.

Mentioned in this Paper

DRUG Screen Quant Caffeine
Specimen Type - Fibroblasts
Simplexvirus
DNA, Viral
Base Excision Repair
Recombination, Genetic
Neonatal Herpes
Genetic Markers
Dose-Response Relationship, Radiation
UV-SENSITIVE Syndrome 1

About this Paper

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