PMID: 7029464Aug 25, 1981Paper

Genetic recombination: recA protein promotes homologous pairing between duplex DNA molecules without strand unwinding

Nucleic Acids Research
E CassutoP Howard-Flanders

Abstract

The recA protein of Escherichia coli promotes pairing in vitro between covalent circular duplex DNA and homologous circular duplex DNA containing a single stranded region. We have used a filter binding assay to investigate the frequency of homologous pairing between gapped and intact duplex DNA when unwinding of the free 3' and 5' ends of the gapped molecules was blocked. In order to obtain DNA without free ends, the gapped DNA was treated with trimethylpsoralen and 360 nm light so as to introduce about 6 crosslinks per DNA molecule and the double stranded regions on either side of the gaps were then digested up to the first crosslinks with exonuclease III and lambda exonuclease. This treatment did not diminish the frequency of homologous pairing, an observation which is difficult to reconcile with models for recombination requiring strand unwinding before pairing.

References

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Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J H Wilson
Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E CassutoP Howard-Flanders
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May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S C WestP Howard-Flanders
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·E CassutoP Howard-Flanders
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S C WestP Howard-Flanders

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Citations

Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H J LippsD M Prescott
Nov 11, 1983·Nucleic Acids Research·D F DombroskiA R Morgan
Mar 5, 2011·Microbiology and Molecular Biology Reviews : MMBR·Jeremy J GilbreathDavid R Hendrixson
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·G Yagil
Jun 10, 2015·Biosensors & Bioelectronics·Jonathan Sabaté Del RíoCiara K O'Sullivan
Jan 12, 2007·Molecular BioSystems·Indraneel GhoshDavid J Segal
Apr 27, 2017·Briefings in Functional Genomics·Samuel Corless, Nick Gilbert

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