Genetic redundancy in the catabolism of methylated amines in the yeast Scheffersomyces stipitis

Antonie van Leeuwenhoek
Tomas Linder

Abstract

The catabolism of choline as a source of nitrogen in budding yeasts is thought to proceed via the intermediates trimethylamine, dimethylamine and methylamine before the release of ammonia. The present study investigated the utilisation of choline and its downstream intermediates as nitrogen sources in the yeast Scheffersomyces stipitis using a reverse genetics approach. Six genes (AMO1, AMO2, SFA1, FGH1, PICST_49761, PICST_63000) that have previously been predicted to be directly or indirectly involved in the catabolism of methylated amines were individually deleted. The growth of each deletion mutant was assayed on minimal media with methylamine, dimethylamine, trimethylamine or choline as the sole nitrogen source. The two amine oxidase-encoding genes AMO1 and AMO2 appeared to be functionally redundant for growth on methylated amines as both deletion mutants displayed growth on all nitrogen sources tested. However, deletion of AMO1 resulted in a pronounced growth lag on all four methylated amines while deletion of AMO2 only caused a growth lag when methylamine was the sole nitrogen source. The glutathione-dependent formaldehyde dehydrogenase-encoding gene SFA1 was found to be absolutely essential for growth on all methylated a...Continue Reading

References

Jan 1, 1981·Archives of Microbiology·J P van Dijken, P Bos
Jan 1, 1962·Antonie van Leeuwenhoek·J van der WALT
Dec 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jonas WarringerAnders Blomberg
Jan 22, 2005·Nucleic Acids Research·Kazutaka KatohTakashi Miyata
Sep 30, 2006·Biochimica Et Biophysica Acta·Cécile Brocard, Andreas Hartig
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar

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Datasets Mentioned

BETA
Y14837

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BETA
PCR
transgenic
deamination

Software Mentioned

FigTree
TBLASTN
MEGA

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