PMID: 2118503Sep 1, 1990Paper

Genetic regulation of formate hydrogenlyase of Escherichia coli: role of the fhlA gene product as a transcriptional activator for a new regulatory gene, fhlB

Journal of Bacteriology
J A Maupin, K T Shanmugam

Abstract

A new gene whose product is required for the production of formate hydrogenlyase (FHL) has been identified in Escherichia coli. This gene, termed fhlB, maps between the frdA (94.4 min) and argI (96.6 min) genes on the E. coli chromosome and is transcribed in a clockwise direction toward argI. Biochemical analysis of an FhlB- mutant, strain SE-2011 [phi(fhlB-lacZ+)], revealed that the mutant lacks formate dehydrogenase activity associated with FHL (FDH-H) and hydrogenase activity. As a result of these defects, fermentative hydrogen production and hydrogen uptake reactions were undetectable in strain SE-2011. Fumarate reductase activity of this mutant was also reduced to about 15% of the levels of the parent (strain MC4100), and strain SE-2011 did not produce succinate as a fermentation end product. Regulation of expression of the fhlB gene, studied as production of beta-galactosidase activity by strain SE-2011, revealed that the operon is expressed at low levels under aerobic conditions. Under anaerobic growth conditions, this activity increased by two- to threefold. Addition of formate enhanced the differential rate of synthesis of the fhlB gene product to as high as 130 U of beta-galactosidase specific activity per microgram o...Continue Reading

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Citations

Feb 9, 2012·Critical Reviews in Biochemistry and Molecular Biology·Karen TrchounianArmen Trchounian
Nov 23, 2015·Applied Biochemistry and Biotechnology·Muhammad JawedYunjun Yan
Jan 1, 1994·Antonie van Leeuwenhoek·G Sawers
Jul 1, 1993·Molecular Microbiology·S Iuchi, E C Lin
Feb 13, 2009·PloS One·Zhanmin FanRanjini Chatterjee
Aug 19, 2006·Canadian Journal of Microbiology·Richard SparlingDavid B Levin
Mar 1, 1993·Journal of Bacteriology·J YangA J Pittard
Oct 1, 1994·Journal of Bacteriology·D Foster-HartnettR G Kranz
Aug 1, 1997·Journal of Bacteriology·S ChattopadhyayP Datta
Sep 1, 1995·Journal of Bacteriology·J A Maupin-FurlowK T Shanmugam
Sep 1, 1994·Microbiological Reviews·H M Fischer
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
Dec 1, 1993·Microbiological Reviews·M Riley

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