Genetic Regulation of Guanylate-Binding Proteins 2b and 5 during Leishmaniasis in Mice

Frontiers in Immunology
Yahya SohrabiMarie Lipoldová

Abstract

Interferon-induced GTPases [guanylate-binding proteins (GBPs)] play an important role in inflammasome activation and mediate innate resistance to many intracellular pathogens, but little is known about their role in leishmaniasis. We therefore studied expression of Gbp2b/Gbp1 and Gbp5 mRNA in skin, inguinal lymph nodes, spleen, and liver after Leishmania major infection and in uninfected controls. We used two different groups of related mouse strains: BALB/c, STS, and CcS-5, CcS-16, and CcS-20 that carry different combinations of BALB/c and STS genomes, and strains O20, C57BL/10 (B10) and B10.O20, OcB-9, and OcB-43 carrying different combinations of O20 and B10 genomes. The strains were classified on the basis of size and number of infection-induced skin lesions as highly susceptible (BALB/c, CcS-16), susceptible (B10.O20), intermediate (CcS-20), and resistant (STS, O20, B10, OcB-9, OcB-43). Some uninfected strains differed in expression of Gbp2b/Gbp1 and Gbp5, especially of Gbp2b/Gbp1 in skin. Uninfected BALB/c and STS did not differ in their expression, but in CcS-5, CcS-16, and CcS-20, which all carry BALB/c-derived Gbp gene-cluster, expression of Gbp2b/Gbp1 exceeds that of both parents. These data indicate trans-regulation ...Continue Reading

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S L GuptaS L Holmes
Apr 1, 1996·The Journal of Experimental Medicine·S StengerC Bogdan
Jan 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·A P StassenP Demant
Jun 20, 2002·Immunogenetics·Marie LipoldováPeter Demant
Mar 26, 2004·Nature Reviews. Molecular Cell Biology·Gerrit J K Praefcke, Harvey T McMahon
Mar 18, 2006·Nature Reviews. Genetics·Marie Lipoldová, Peter Demant
Jun 20, 2006·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Keith R Shockley, Gary A Churchill
Aug 13, 2008·International Journal of Cancer. Journal International Du Cancer·Elisabeth NaschbergerMichael Stürzl
Jun 12, 2010·Nature Protocols·Tetyana KobetsMarie Lipoldová
Aug 19, 2010·Journal of Cellular and Molecular Medicine·Matthias HammonElisabeth Naschberger
Dec 15, 2010·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Deborah J Vestal, Jonathan A Jeyaratnam
Jun 15, 2011·PLoS Neglected Tropical Diseases·Matyáš SímaMarie Lipoldová
Sep 6, 2011·Journal of Microbiological Methods·Igor GrekovMarie Lipoldová
Nov 23, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alexandra NordmannStephan Ludwig
Jun 14, 2012·PloS One·Jorge AlvarUNKNOWN WHO Leishmaniasis Control Team
Dec 19, 2012·Proceedings of the National Academy of Sciences of the United States of America·Daniel DegrandiKlaus Pfeffer
Jul 23, 2013·PLoS Neglected Tropical Diseases·Yahya SohrabiMarie Lipoldová
Nov 18, 2015·Cellular Microbiology·Etienne Meunier, Petr Broz
Mar 22, 2016·Cell Host & Microbe·Christian KrappFrank Kirchhoff
Apr 20, 2016·Nature Immunology·Bae-Hoon KimJohn D MacMicking
May 18, 2016·Journal of Molecular Biology·Danielle Pilla-MoffettJörn Coers
Jun 14, 2016·Nature Reviews. Immunology·Petr Broz, Vishva M Dixit
Jul 16, 2016·Journal of Leukocyte Biology·Si Ming ManThirumala-Devi Kanneganti
Jan 18, 2017·Proceedings of the National Academy of Sciences of the United States of America·Michael A Mandell, Stephen M Beverley

❮ Previous
Next ❯

Methods Mentioned

BETA
GTPase
biopsies
PCR
ELISA
dissection
genotyping

Software Mentioned

KIM
ProbeFinder
Statistica

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.