PMID: 5903090Jan 1, 1966

Genetic relatedness among mycoplasmas as determined by nucleic acid homology

Journal of Bacteriology
P R ReichS M Weissman

Abstract

Reich, Paul R. (National Institutes of Health, Bethesda, Md.), Norman L. Somerson, James A. Rose, and Sherman M. Weissman. Genetic relatedness among mycoplasmas as determined by nucleic acid homology. J. Bacteriol. 91:153-160. 1966.-A sensitive membrane filter method to detect nucleic acid homology was used to determine genetic relatedness among mycoplasma isolates. Deoxyribonucleic acid (DNA) was isolated from mycoplasmas and used as a primer for synthesis of tritium-labeled, complementary ribonucleic acid (RNA) by the enzyme RNA polymerase. DNA from each mycoplasma isolate tested was reacted separately with complementary RNA synthesized with homologous or heterologous DNA as primer. The quantity of DNA-RNA hybrids formed was assayed by the nitrocellulose membrane filter method. The amount of radioactivity bound to the membrane filter was used to measure the degree of homology between the nucleic acids. The three mycoplasma isolates from human oral cavities (DC 63, V2785, Botteicher) and the prototype strain PG21 placed in the Mycoplasma hominis type 1 group by gel diffusion and complement-fixation testing were investigated with this technique. Analysis of the data confirmed their immunological grouping with the M. hominis typ...Continue Reading

Citations

Mar 31, 1967·Science·D G EdwardR G WITTLER
Jul 28, 1967·Annals of the New York Academy of Sciences·Z A McGeeR G WITTLER
Jul 28, 1967·Annals of the New York Academy of Sciences·N L SOMERSONS M Weissman
Oct 30, 1970·Annals of the New York Academy of Sciences·A Martinez-LahozL HAYFLICK
Feb 3, 2007·International Journal of Systematic and Evolutionary Microbiology·Robert F Whitcomb
Jul 28, 1967·Annals of the New York Academy of Sciences·R H PURCELLJ Valdesuso

Related Concepts

DNA, Bacterial
Science of Genetics
Mycoplasma putrefaciens
RNA, Bacterial
Tritium

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