PMID: 4337161May 1, 1972Paper

Genetic relatedness of type 1 and type 2 herpes simplex viruses

Journal of Virology
E KieffB Roizman


The extent of homology between herpes simplex virus(1) and(2) (HSV-1 and HSV-2) deoxyribonucleic acid (DNA) was measured in two ways: (i) by determination of the relative rate of hybridization of labeled HSV-1 and HSV-2 DNA to excess unlabeled HSV-1 or HSV-2 DNA immobilized on filters and (ii) by determination of the rate of hybridization of labeled HSV-1 and HSV-2 DNA to excess unlabeled HSV-1 or HSV-2 DNA in solution. Approximately 40% of HSV-1 and HSV-2 DNA is homologous at hybridization temperatures 25 C below the melting temperature (T(m)) of HSV DNA (liquid-filter annealing). Lowering the temperature to 34 C below the T(m) increased the extent of homology to 46% (liquid annealing). The extent of base-pairing in HSV-1-HSV-2 heteroduplex DNA was determined by thermal chromatography on hydroxyapatite. Heteroduplexes of HSV-1 and HSV-2 DNA eluted in a single peak whose midpoint (Te(50)) was 10 C below that of the homoduplex. Conspicuously absent were heteroduplexes that eluted at more than 15 C below the Te(50) of the homoduplex. The data indicate the existence of a variable region of DNA (54%) with very little, if any, homology and an invariable region (46%) with relatively good (85%) matching of base pairs.


Sep 19, 1975·Medical Microbiology and Immunology·H Schulte-Holthausen, K E Schneweis
Jan 1, 1991·Archives of Virology·H Piiparinen, A Vaheri
Jan 22, 2020·The Journal of General Virology·Oskar BunzAnja Ehrhardt
Oct 1, 1975·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·A VahlneE Lycke
Jan 1, 1980·Annals of the New York Academy of Sciences·E S HuangC Alford
Jan 1, 1979·Microbiology and Immunology·A Tanaka, Y Numazaki
Jan 1, 1979·International Journal of Dermatology·R Giacobetti
Sep 1, 1979·Journal of Clinical Pathology·M C Timbury, E Edmond
Jan 1, 1980·Annals of the New York Academy of Sciences·T G BuchmanA J Nahmias
Jan 1, 1980·Archives of Virology·A EpsteinJ Huppert
Apr 1, 1984·Postgraduate Medical Journal·P G Kennedy
Nov 12, 2005·Current Eye Research·David M Koelle, Homayon Ghiasi
Feb 27, 2009·Archives of Gynecology and Obstetrics·Işil MaralM Ali Bumin
Sep 27, 1973·The New England Journal of Medicine·A J Nahmias, B Roizman
Dec 1, 1995·Immunology and Cell Biology·C W Schmidt, I S Misko
Aug 1, 2020·Cornea·James Chodosh, Lawson Ung
Dec 1, 1977·Journal of Virology·G P AllenC C Randall
Jun 1, 1983·Journal of Virology·M A Swain, D A Galloway
Jun 1, 1976·Journal of Virology·B A KilpatrickJ S Pagano
May 1, 1980·Journal of Virology·G H CohenR J Eisenberg
Jan 1, 1980·Journal of Immunological Methods·C Y Ching, C López

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology
L XieW Shi
International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases
Hind A BeydounMay A Beydoun
Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie
Hua Yan, Jun Li
© 2021 Meta ULC. All rights reserved