Genetic relationships among strains of Xanthomonas fragariae based on random amplified polymorphic DNA PCR, repetitive extragenic palindromic PCR, and enterobacterial repetitive intergenic consensus PCR data and generation of multiplexed PCR primers useful for the identification of this phytopathogen.

Applied and Environmental Microbiology
M R PoolerJ S Hartung

Abstract

Genetic relationships among 25 isolates of Xanthomonas fragariae from diverse geographic regions were determined by three PCR methods that rely on different amplification priming strategies: random amplified polymorphic DNA (RAPD) PCR, repetitive extragenic palindromic (REP) PCR, and enterobacterial repetitive intergenic consensus (ERIC) PCR. The results of these assays are mutually consistent and indicate that pathogenic strains are very closely related to each other. RAPD, ERIC, and REP PCR assays identified nine, four, and two genotypes, respectively, within X. fragariae isolates. A single nonpathogenic isolate of X. fragariae was not distinguishable by these methods. The results of the PCR assays were also fully confirmed by physiological tests. There was no correlation between DNA amplification product patterns and geographic sites of isolation, suggesting that this bacterium has spread largely through exchange of infected plant germ plasm. Sequences identified through the RAPD assays were used to develop three primer pairs for standard PCR assays to identify X. fragariae. In addition, we developed a stringent multiplexed PCR assay to identify X. fragariae by simultaneously using the three independently derived sets of pri...Continue Reading

References

Aug 1, 1991·PCR Methods and Applications·T BarryF Gannon
Nov 25, 1990·Nucleic Acids Research·J G WilliamsS V Tingey
Aug 1, 1995·Current Microbiology·M R Pooler, J S Hartung
Sep 1, 1993·Applied and Environmental Microbiology·L M LawrenceA Gilmour
Jan 1, 1993·Annual Review of Phytopathology·J M Henson, R French

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Citations

Mar 17, 2004·International Journal of Systematic and Evolutionary Microbiology·Jaime Cubero, James H Graham
Mar 7, 2003·Applied and Environmental Microbiology·Dhruva BhattacharyaBanwari Lal
Feb 26, 2016·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Jack A RoachVance M Whitaker
Mar 12, 2004·Journal of Applied Microbiology·A GonzálezJ M Guillamón
Feb 8, 2006·Applied and Environmental Microbiology·Isabelle Robène-SoustradeOlivier Pruvost
Jan 4, 2018·Phytopathology·Hehe WangWilliam W Turechek
Jun 29, 2002·Applied and Environmental Microbiology·Yasuhiro OdaLarry J Forney
Nov 27, 2020·Scientific Reports·Joanna PuławskaJan M van der Wolf
Apr 24, 2021·Microbiology Resource Announcements·Austin HerbertWilliam Turechek

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