Genetic requirements for growth of Escherichia coli K12 on methyl-alpha-D-glucopyranoside and the five alpha-D-glucosyl-D-fructose isomers of sucrose

The Journal of Biological Chemistry
Andreas PikisJohn Thompson

Abstract

Strains of Escherichia coli K12, including MG-1655, accumulate methyl-alpha-D-glucopyranoside via the phosphoenolpyruvate-dependent glucose:phosphotransferase system (IICB(Glc)/IIA(Glc)). High concentrations of intracellular methyl-alpha-D-glucopyranoside 6-phosphate are toxic, and cell growth is prevented. However, transformation of E. coli MG-1655 with a plasmid (pAP1) encoding the gene aglB from Klebsiella pneumoniae resulted in excellent growth of the transformant MG-1655 (pAP1) on the glucose analog. AglB is an unusual NAD+/Mn2+-dependent phospho-alpha-glucosidase that promotes growth of MG-1655 (pAP1) by catalyzing the in vivo hydrolysis of methyl-alpha-D-glucopyranoside 6-phosphate to yield glucose 6-phosphate and methanol. When transformed with plasmid pAP2 encoding the K. pneumoniae genes aglB and aglA (an alpha-glucoside-specific transporter AglA (IICB(Agl))), strain MG-1655 (pAP2) metabolized a variety of other alpha-linked glucosides, including maltitol, isomaltose, and the following five isomers of sucrose: trehalulose alpha(1-->1), turanose alpha(1-->3), maltulose alpha(1-->4), leucrose alpha(1-->5), and palatinose alpha(1-->6). Remarkably, MG-1655 (pAP2) failed to metabolize sucrose alpha(1-->2). The E. coli K12 ...Continue Reading

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Citations

Jan 11, 2012·Molecular Oral Microbiology·J Thompson, A Pikis
Dec 22, 2012·Beneficial Microbes·M Rothe, M Blaut
Mar 16, 2017·Journal of Bacteriology·Chelsea R LloydCarin K Vanderpool
Jul 23, 2013·Journal of Bacteriology·Yan Sun, Carin K Vanderpool
Mar 3, 2019·Molecular Biology and Evolution·Otmane LamrabetThomas Hindré
Jan 14, 2021·Microbial Biotechnology·Pauline L FolchRuud A Weusthuis
Jun 13, 2021·Proceedings of the National Academy of Sciences of the United States of America·Handuo ShiPetra Anne Levin
Aug 17, 2011·Biochimica Et Biophysica Acta·Gregory R Richards, Carin K Vanderpool

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