Genetic screen for suppressors of increased silencing in rpd3 mutants in Saccharomyces cerevisiae identifies a potential role for H3K4 methylation.

G3 : Genes - Genomes - Genetics
Richard A KleinschmidtDavid Donze

Abstract

Several studies have identified the paradoxical phenotype of increased heterochromatic gene silencing at specific loci that results from deletion or mutation of the histone deacetylase (HDAC) gene RPD3. To further understand this phenomenon, we conducted a genetic screen for suppressors of this extended silencing phenotype at the HMR locus in Saccharomyces cerevisiae. Most of the mutations that suppressed extended HMR-silencing in rpd3 mutants without completely abolishing silencing were identified in the histone H3 lysine 4 methylation (H3K4me) pathway, specifically in SET1, BRE1 and BRE2. These second site mutations retained normal HMR silencing, therefore appear to be specific for the rpd3Δ extended silencing phenotype. As an initial assessment of the role of H3K4 methylation in extended silencing, we rule out some of the known mechanisms of Set1p/H3K4me mediated gene repression by HST1, HOS2 and HST3 encoded HDACs. Interestingly, we demonstrate that the RNA Polymerase III complex remains bound and active at the HMR-tDNA in rpd3 mutants despite silencing extending beyond the normal barrier. We discuss these results as they relate to the interplay among different chromatin modifying enzyme functions and the importance of furt...Continue Reading

References

Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L M JohnsonM Grunstein
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·S E RundlettM Grunstein
Jan 7, 1997·Proceedings of the National Academy of Sciences of the United States of America·P Ross-MacdonaldM Snyder
Mar 18, 1999·Current Genetics·E G DoraR M Ramírez
Jun 1, 1999·Methods in Enzymology·P Ross-MacdonaldM Snyder
Nov 1, 2001·Proceedings of the National Academy of Sciences of the United States of America·T MillerA Shilatifard
Jun 19, 2002·The Journal of Biological Chemistry·Jim DoverAli Shilatifard
Jul 23, 2002·Molecular Biology of the Cell·Laura N RuschéJasper Rine
Aug 2, 2002·Nature·Scott D BriggsBrian D Strahl
Jan 1, 1956·Cold Spring Harbor Symposia on Quantitative Biology·H ROMAN
May 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·V G ALLFREYA E MIRSKY
Jul 29, 2004·The Journal of Biological Chemistry·Helena Santos-RosaTony Kouzarides
Sep 9, 2008·Molecular Cell·Chia-Ching ChouMarc R Gartenberg
Apr 18, 2009·Nucleic Acids Research·Jing ZhouJin-Qiu Zhou
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Stefan EhrentrautAnn E Ehrenhofer-Murray
Nov 24, 2011·Nucleic Acids Research·J Michael CherryEdith D Wong
Jan 3, 2014·The Journal of Clinical Investigation·Alison C West, Ricky W Johnstone
Dec 24, 2015·Genomics Data·Jeffrey N McKnight, Toshio Tsukiyama
Jun 28, 2016·Nature Reviews. Genetics·C David Allis, Thomas Jenuwein
May 19, 2019·The Journal of Biological Chemistry·Aseem Z Ansari
Aug 31, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tasha B Toro, Terry J Watt
Sep 23, 2020·Nature Reviews. Genetics·Bercin K Cenik, Ali Shilatifard
Oct 21, 2020·Proceedings of the National Academy of Sciences of the United States of America·Daniel S Saxton, Jasper Rine
Jan 8, 2021·ACS Chemical Biology·Roberto Di BlasiHarry J Whitwell
Jan 21, 2021·Nature Reviews. Drug Discovery·Kamakoti P BhatOr Gozani
Mar 12, 2021·Seminars in Cancer Biology·Fabin Dang, Wenyi Wei

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.