Genetic signatures of high- and low-risk aberrant crypt foci in a mouse model of sporadic colon cancer

Cancer Research
Prashant R NambiarDaniel W Rosenberg

Abstract

To determine whether cancer risk is related to histopathological features of preneoplastic aberrant crypt foci (ACF), gene expression analysis was performed on ACF from two mouse strains with differing tumor sensitivity to the colonotropic carcinogen, azoxymethane. ACF from sensitive A/J mice were considered at high risk, whereas ACF from resistant AKR/J mice were considered at low risk for tumorigenesis. A/J and AKR/J mice received weekly injections of azoxymethane (10 mg/kg body weight), and frozen colon sections were prepared 6 weeks later. Immunohistochemistry was performed using biomarkers associated with colon cancer, including adenomatous polyposis coli, beta-catenin, p53, c-myc, cyclin D1, and proliferating cell nuclear antigen. Hyperplastic ACF, dysplastic ACF, microadenomas, adjacent normal-appearing epithelium, and vehicle-treated colons were laser captured, and RNA was linearly amplified (LCM-LA) and subjected to cDNA microarray-based expression analysis. Patterns of gene expression were identified using adaptive centroid algorithm. ACF from low- and high-risk colons were not discriminated by immunohistochemistry, with the exception of membrane staining of beta-catenin. To develop genetic signatures that predict can...Continue Reading

References

Jun 1, 1990·Cell·E R Fearon, B Vogelstein
Jul 1, 1996·Diseases of the Colon and Rectum·B ShpitzH Stern
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·K L Neufeld, R L White
Feb 5, 1998·Carcinogenesis·T MaltzmanD Ahnen
Oct 29, 1998·The New England Journal of Medicine·T TakayamaY Niitsu
Apr 14, 1999·CA: a Cancer Journal for Clinicians·S H LandisP A Wingo
Dec 10, 1999·Toxicologic Pathology·C M Fenoglio-Preiser, A Noffsinger
Jan 15, 2000·Scandinavian Journal of Gastroenterology·J KristinssonM K Fagerhol
Mar 4, 2000·The Journal of Pathology·J W Arends
Aug 15, 2000·Molecular Carcinogenesis·Q S WangD W Rosenberg
Jul 17, 2001·Histochemistry and Cell Biology·K A FeldmannJ P Grande
Oct 2, 2001·Journal of Gastroenterology·T FujimoriH Kashida
Jun 28, 2002·Cancer Cell·Sanford D MarkowitzJames K V Willson
Jul 13, 2002·Trends in Molecular Medicine·Kaichun WeiWinfried Edelmann
Nov 16, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Jiadi HuDhananjaya V Kalvakolanu
Apr 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Xiao-Jun MaDennis C Sgroi
May 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Nick DevoogdtHilde Revets
Jun 5, 2003·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·A. G RenehanC. S Potten
Aug 2, 2003·Biotechnology Progress·Sanjeev GargLuke E K Achenie
Jul 17, 2008·Comparative and Functional Genomics·Olaf WolkenhauerFatima Sanchez-Cabo

❮ Previous
Next ❯

Citations

Sep 22, 2012·Seminars in Immunopathology·Masako Nakanishi, Daniel W Rosenberg
Dec 11, 2008·Cancer Causes & Control : CCC·Helen SwedeDaniel W Rosenberg
Jan 24, 2009·Molecular & Cellular Proteomics : MCP·Baogang J XuRichard M Caprioli
Mar 4, 2006·Carcinogenesis·Emily J GreenspanDaniel W Rosenberg
Nov 28, 2008·Carcinogenesis·Daniel W RosenbergTakuji Tanaka
May 18, 2011·Cancer Prevention Research·Masako NakanishiDaniel W Rosenberg
Jul 23, 2011·Genes & Cancer·Rónán C O'Hagan, Joerg Heyer
Nov 21, 2007·Annals of Surgical Oncology·Tetsuro SasadaArimichi Takabayashi
Jan 1, 2008·Clinical and Experimental Gastroenterology·Claire M PayneHarris Bernstein
Sep 25, 2014·The Journal of Clinical Investigation·Maria Noé GarciaJuan L Iovanna
Feb 6, 2013·Expert Opinion on Therapeutic Targets·Mei X WuOleg E Akilov
Nov 17, 2011·Ultrastructural Pathology·Liping HanMei X Wu
Oct 16, 2015·International Journal of Molecular Medicine·Xuehu XuShangbiao Wu
Nov 30, 2010·European Journal of Cell Biology·Alexander Arlt, Heiner Schäfer
Dec 22, 2006·Cancer Letters·Richard G StevensDaniel W Rosenberg
Aug 12, 2005·International Journal of Cancer. Journal International Du Cancer·Jan Erik PaulsenJan Alexander
Sep 25, 2009·Molecular Carcinogenesis·Scott C BorinsteinWilliam M Grady
Oct 13, 2005·Biochimica Et Biophysica Acta·Theresa P Pretlow, Thomas G Pretlow
Oct 23, 2015·Environmental Toxicology and Pharmacology·Roseane Mendes SilvaNora Manoukian Forones
Mar 30, 2011·Acta Cirúrgica Brasileira·Roberta Alves HigaAlbert Schiaveto de Souza
Nov 9, 2006·Molecular Carcinogenesis·Christopher FlynnDaniel W Rosenberg
Dec 1, 2007·Proteomics. Clinical Applications·Masako NakanishiDaniel W Rosenberg
Feb 14, 2008·International Journal of Cancer. Journal International Du Cancer·Shelly R CalcagnoNicole R Murray
Jul 16, 2008·Journal of Surgical Oncology·Frank A OrlandoSadir J Alrawi
Feb 13, 2018·The Journal of Obstetrics and Gynaecology Research·Yuan LiLiping Han
Feb 17, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Zhong LiuJun Liang
May 3, 2008·Cancer Research·Masako NakanishiDaniel W Rosenberg
Aug 3, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Maryla KrajewskaJohn C Reed

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adenomatous Polyposis Coli

Adenomatous polyposis coli is a protein encoded by the APC gene and acts as a tumor suppressor. Discover the latest research on adenomatous polyposis coli here.