PMID: 9537035Jan 1, 1980Paper

Genetic specificity of primary and secondary proliferative and cytotoxic responses of human lymphocytes grown in continued culture

Scandinavian Journal of Immunology
D J SchendelG D Bonnard

Abstract

Human T peripheral blood lymphocytes were grown in continued culture using conditioned medium obtained from phytohaemagglutinin-stimulated, pooled human leucocytes. These cultured T cells (CTC) were tested in mixed lymphocyte culture (MLC) and cell-mediated lympholysis (CML) assays to determine the genetic specificity of their proliferative and cytotoxic responses. Primary responses were measured after initial in vitro stimulation by allogeneic cells, and secondary responses were measured after a second in vitro stimulation by allogeneic cells. Both primary and secondary proliferative responses were found to be stimulated by alloantigens controlled by the HLA region and, more specifically, by antigens of the HLA-D region, in accordance with the responses of normal peripheral blood T lymphocytes. When CTC were established from unsensitized PBL and then stimulated with allogeneic cells, they could respond by proliferation in MLC, but, in contrast to PBL, they did not show subsequent cytotoxic responses. On the other hand, CTC established from PBL that had been stimulated first with allogeneic cells in either primary or secondary MLC displayed high levels of cytotoxic reactivity in CML. The strongest cytotoxicity was directed agai...Continue Reading

References

Oct 21, 1976·The New England Journal of Medicine·F H Bach, J J van Rood
Sep 1, 1975·Scandinavian Journal of Immunology·K S Zier, F H Bach
Jul 14, 1977·Nature·S Gillis, K A Smith
Feb 1, 1979·Tissue Antigens·D J SchendelB Dupont
Oct 1, 1978·The Journal of Experimental Medicine·S GillisK A Smith

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Citations

Jul 1, 1981·Journal of Clinical Immunology·J B BurnsR P Lisak
Jul 1, 1981·Human Immunology·D J Schendel, R Wank
Apr 1, 1994·The Journal of Experimental Medicine·E P Rieber, G Rank
Oct 1, 1989·Blut·A LindemannR Mertelsmann
Jul 1, 1981·Tissue Antigens·T KristensenC Mawas
Jan 1, 1981·Immunological Reviews·J KornbluthB Dupont
Jun 1, 1987·Pediatric Annals·R B Herberman
Jan 15, 1982·International Journal of Cancer. Journal International Du Cancer·B M Vose, G D Bonnard
Feb 1, 1981·Tissue Antigens·N MorlingM Thomsen
Dec 1, 1991·Immunological Investigations·U ShahK S Zier

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