Genetic stability of Sabin 1 strain of poliovirus: implications for quality control of oral poliovirus vaccine

Virology
G V RezapkinK M Chumakov

Abstract

The Sabin vaccine strains of poliovirus, like all RNA viruses, exist as a quasispecies of genomic sequences whose composition can be altered during virus propagation. Since changes in vaccine virus during manufacture can enhance the neurovirulent potential of the vaccine, each monovalent lot of oral poliovirus vaccine (OPV) undergoes several tests to ensure consistency of manufacture, including the monkey neurovirulence test (MNVT). Recently, we proposed a new molecular approach for direct quantification of vaccine variants with neurovirulent potential as an alternative way to monitor consistency of OPV production. Analysis of the Sabin 1 genome allowed us to identify a limited number of specific loci that exhibit significant change during viral propagation in vitro and in vivo. Here we explore the possible roles of these changes and show that 7427-U-->C and 7441-G-->A alterations in the 3'-UTR of the Sabin 1 virus do not increase monkey neurovirulence. These, as well as our previous results, suggest that only mutations in the 5'-UTR play a significant role in the limited increase in Sabin 1 monkey neurovirulence observed after extended propagation of the virus beyond the passage level used in vaccine production. Our studies wi...Continue Reading

References

Feb 1, 1992·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P M StrebelA R Hinman
Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K M ChumakovI S Levenbook
Mar 1, 1989·Journal of Virology·G D WestropG C Schild
Jul 13, 1957·Journal of the American Medical Association·A B SABIN
Apr 22, 1961·JAMA : the Journal of the American Medical Association·A B SABIN

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