Apr 23, 2020

HSV Forms an HCMV-like Viral Assembly Center in Neuronal Cells

BioRxiv : the Preprint Server for Biology
S. WhiteRichard J Roller

Abstract

Herpes simplex virus (HSV) is a neuroinvasive virus that has been used as a model organism for studying common properties of all herpesviruses. HSV induces host organelle rearrangement and forms dispersed assembly compartments in epithelial cells, which complicates the study of HSV assembly. In this study, we show that HSV forms a visually distinct unitary cytoplasmic viral assembly center (cVAC) in both cancerous and primary neuronal cells that concentrates viral structural proteins and is the site of capsid envelopment. The HSV cVAC also concentrates host membranes that are important for viral assembly, such as Golgi- and recycling endosome-derived membranes. Lastly, we show that HSV cVAC formation and/or maintenance depends on an intact microtubule network and a viral tegument protein, pUL51. Our observations suggest that the neuronal cVAC is a uniquely useful model to study common herpesvirus assembly pathways, and cell-specific pathways for membrane reorganization.

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Mentioned in this Paper

Genetic Drift
Genome
Genes
Spatial Distribution
Loss of Heterozygosity
Genomics
Cell Growth
Alleles
Gene Mutant
Mutation Abnormality

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