Genetic tagging of active neurons in auditory cortex reveals maternal plasticity of coding ultrasonic vocalizations

Nature Communications
Gen-Ichi TasakaAdi Mizrahi

Abstract

Cortical neurons are often functionally heterogeneous even for molecularly defined subtypes. In sensory cortices, physiological responses to natural stimuli can be sparse and vary widely even for neighboring neurons. It is thus difficult to parse out circuits that encode specific stimuli for further experimentation. Here, we report the development of a Cre-reporter mouse that allows recombination for cellular labeling and genetic manipulation, and use it with an activity-dependent Fos-CreERT2 driver to identify functionally active circuits in the auditory cortex. In vivo targeted patch recordings validate our method for neurons responding to physiologically relevant natural sounds such as pup wriggling calls and ultrasonic vocalizations (USVs). Using this system to investigate cortical responses in postpartum mothers, we find a transient recruitment of neurons highly responsive to USVs. This subpopulation of neurons has distinct physiological properties that improve the coding efficiency for pup USV calls, implicating it as a unique signature in parental plasticity.

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Citations

Apr 23, 2020·American Journal of Physiology. Cell Physiology·Juliette E Cheyne, Johanna M Montgomery
Aug 1, 2018·Cell and Tissue Research·Silvana Valtcheva, Robert C Froemke
Feb 13, 2020·Frontiers in Neural Circuits·Ido MaorAdi Mizrahi
Nov 19, 2020·Nature Communications·H Shani-NarkissA Mizrahi
Sep 24, 2020·Journal of Neurophysiology·Bau N PhamV Reggie Edgerton
Jun 14, 2019·Neuroscience and Biobehavioral Reviews·Morgan M Rogers-Carter, John P Christianson
May 28, 2021·The European Journal of Neuroscience·Parker K StevensonKeerthi Krishnan

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Methods Mentioned

BETA
transgenic
nuclear translocation
transfection
PCR
chip

Software Mentioned

Photoshop
USV
MATLAB
TRAPing
TRAP
ImageJ Plugin ‘ Simple Neurite Tracer
ImageJ
imtophat
TRAPed
imageJ Plugin ‘ Cell Counter ’

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