Genetic tailoring of N-linked oligosaccharides: the role of glucose residues in glycoprotein processing of Saccharomyces cerevisiae in vivo

Glycobiology
C A JakobJürgen Roth

Abstract

In higher eukaryotes a quality control system monitoring the folding state of glycoproteins is located in the ER and is composed of the proteins calnexin, calreticulin, glucosidase II, and UDP-glucose: glycoprotein glucosyltransferase. It is believed that the innermost glucose residue of the N- linked oligosaccharide of a glycoprotein serves as a tag in this control system and therefore performs an important function in the protein folding pathway. To address this function, we constructed Saccharomyces cerevisiae strains which contain nonglucosylated (G0), monoglucosylated (G1), or diglucosylated (G2) glycoproteins in the ER and used these strains to study the role of glucose residues in the ER processing of glycoproteins. These alterations of the oligosaccharide structure did not result in a growth phenotype, but the induction of the unfolded protein response upon treatment with DTT was much higher in G0 and G2 strains as compared to wild-type and G1 strains. Our results provide in vivo evidence that the G1 oligosaccharide is an active oligosaccharide structure in the ER glycoprotein processing pathway of S.cerevisiae. Furthermore, by analyzing N- linked oligosaccharides of the constructed strains we can directly show that no ...Continue Reading

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Citations

Dec 14, 2007·Histochemistry and Cell Biology·Jürgen RothChristian Zuber
Feb 23, 2011·Molecules and Cells·Jürgen RothJin Won Cho
Dec 19, 2003·Protein Expression and Purification·Amirreza Faridmoayer, Christine H Scaman
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Jun 23, 2009·Glycobiology·Robert P QuinnColin J Stirling
Jun 13, 2012·Microbiology and Molecular Biology Reviews : MMBR·Jacob VergheseKevin A Morano
Nov 20, 2002·The Journal of Clinical Investigation·Randal J Kaufman
Feb 13, 2001·Biochemical and Biophysical Research Communications·M ZiakJ Roth
Dec 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Manasi ChavanWilliam Lennarz
Sep 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·C ZuberJ Roth
Jul 6, 2000·FEBS Letters·H HauriO Nufer
Aug 10, 2001·Fungal Genetics and Biology : FG & B·A ConesaC A van den Hondel
Aug 31, 2000·Annual Review of Biochemistry·A J Parodi
Sep 18, 2014·Bioscience, Biotechnology, and Biochemistry·Hiroyuki AzakamiAkio Kato
Jun 4, 2002·Nature Reviews. Molecular Cell Biology·Randal J KaufmanStacey M Arnold
Nov 3, 2016·Histochemistry and Cell Biology·Jürgen Roth, Christian Zuber
Dec 24, 2005·The Journal of Biological Chemistry·Barrie M WilkinsonColin J Stirling
Oct 12, 2007·Physiological Reviews·Daniel N Hebert, Maurizio Molinari

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