Genetic Targeting of Organ-Specific Blood Vessels

Circulation Research
Wenjuan PuBin Zhou

Abstract

Organs of the body require vascular networks to supply oxygen and nutrients and maintain physiological function. The blood vessels of different organs are structurally and functionally heterogeneous in nature. To more precisely dissect their distinct in vivo function in individual organs, without potential interference from off-site targets, it is necessary to genetically target them in an organ-specific manner. The objective of this study was to generate a genetic system that targets vascular endothelial cells in an organ- or tissue-specific manner and to exemplify the potential application of intersectional genetics for precise, target-specific gene manipulation in vivo. We took advantage of 2 orthogonal recombination systems, Dre-rox and Cre-loxP, to create a genetic targeting system based on intersectional genetics. Using this approach, Cre activity was only detectable in cells that had expressed both Dre and Cre. Applying this new system, we generated a coronary endothelial cell-specific Cre (CoEC-Cre) and a brain endothelial cell-specific Cre (BEC-Cre). Through lineage tracing, gene knockout and overexpression experiments, we demonstrated that CoEC-Cre and BEC-Cre efficiently and specifically target blood vessels in the h...Continue Reading

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Citations

Oct 26, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Sophie PayneAlice Neal
Jul 26, 2019·Arteriosclerosis, Thrombosis, and Vascular Biology·Mingjun Liu, Delphine Gomez
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Oct 20, 2020·Frontiers in Physiology·Irene Garcia-GonzalezRui Benedito
Mar 8, 2021·The Journal of Biological Chemistry·Xueying Tian, Bin Zhou
Aug 3, 2021·Genesis : the Journal of Genetics and Development·Henrik OrtsäterTaija Mäkinen
Aug 14, 2021·Acta Pharmaceutica Sinica. B·Chenglong GaoTao Pang

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