Genetic transformation of the model green alga Chlamydomonas reinhardtii

Methods in Molecular Biology
Juliane NeupertRalph Bock

Abstract

Over the past three decades, the single-celled green alga Chlamydomonas reinhardtii has become an invaluable model organism in plant biology and an attractive production host in biotechnology. The genetic transformation of Chlamydomonas is relatively simple and efficient, but achieving high expression levels of foreign genes has remained challenging. Here, we provide working protocols for algal cultivation and transformation as well as for selection and analysis of transgenic algal clones. We focus on two commonly used transformation methods for Chlamydomonas: glass bead-assisted transformation and particle gun-mediated (biolistic) transformation. In addition, we describe available tools for promoting efficient transgene expression and highlight important considerations for designing transformation vectors.

Citations

Jan 24, 2019·Bioscience, Biotechnology, and Biochemistry·Xiaonan PangDefu Chen
May 30, 2019·Molecular Biotechnology·Vikas Kumar PatelBhaskar Bhadra
Sep 24, 2013·Expert Review of Vaccines·Sergio Rosales-Mendoza
Nov 19, 2015·Frontiers in Plant Science·Emanuel Sanz-LuqueEmilio Fernandez
Aug 15, 2014·World Journal of Microbiology & Biotechnology·Fariba AkbariAhmad Yari Khosroushahi
Sep 25, 2015·The Plant Journal : for Cell and Molecular Biology·Rouhollah BarahimipourRalph Bock
Aug 29, 2018·Nature Communications·Zhichao Li, Ralph Bock

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