PMID: 8582343Sep 1, 1995Paper

Genetic typing with HUMTH01, HUMVWA31A and HUMFES/FPS short tandem repeat loci, D1S80 variable number tandem repeat locus and HLA-DQ alpha of recent and from XII-XIII centuries spongy bone

Electrophoresis
M M de PancorboC de la Rúa

Abstract

The genetic analysis of ancient populations through DNA from bone remains, requires use of short sized loci that can be amplified by polymerase chain reaction (PCR) for which the short tandem repeat (STR) loci are most suitable. These techniques can also be applied to genetic identification in forensic casework. In this study three STR loci, HUMFES/FPS, HUMTH01, and HUMVWA31A, were selected to estimate their usefulness when applied to recent and ancient spongy bone DNA typing. In addition, loci D1S80 and HLA DQ alpha were also tested in the analysis of recent spongy bone DNA. The recent remains studied were constituted by ten spongy bone samples of postmortem material from one individual buried for 1 year. The ancient remains are composed by 8 spongy bone samples from the heads of left femurs from a XII-XIII Centuries Basque Country population. Adequate amplification and typing results could only be obtained with cetyltrimethyl ammonium bromide (CTAB)-extracted DNA, without any further purification after precipitation. Genotypes of the one year post-mortem material and those of his son and his wife were obtained at the D1S80, HLA-DQ alpha, and STR loci. In all these systems, no exclusion was observed, with a combined probabilit...Continue Reading

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Citations

Mar 26, 2003·American Journal of Physical Anthropology·Frederika A Kaestle, K Ann Horsburgh
Jun 5, 2003·Analytical Biochemistry·Biljana CuljkovićStanka Romac
Jan 25, 2012·Journal of Forensic Sciences·Chang Seok OhDong Hoon Shin
May 23, 2015·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Chang Seok OhDong Hoon Shin
Jan 18, 2005·Revista do Hospital das Clínicas·Edna Sadayo Miazato IwamuraDaniel Romero Muñoz

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