Genetic Variability of Chikungunya Virus in Southern Mexico

Viruses
Kame A Galán-HuertaAna M Rivas-Estilla

Abstract

Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that causes Chikungunya fever. CHIKV entered Mexico through the state of Chiapas in October 2014. To fully understand the Chikungunya fever outbreak that occurred in southern Chiapas during 2015, we evaluated 22 PCR-confirmed CHIKV-positive patients, identified CHIKV genetic variability, reconstructed viral dispersal, and assessed possible viral mutations. Viruses were isolated and E2, 6K, and E1 genes were sequenced. We applied phylogenetic and phylogeographic approaches, modeled mutations, and estimated selective pressure. Different CHIKV strains circulated in Chiapas during summer 2015. Three isolates grouped themselves in a well-supported clade. Estimates show that the outbreak started in Ciudad Hidalgo and posteriorly dispersed towards Tapachula and neighboring municipalities. We found six non-synonymous mutations in our isolates. Two mutations occurred in one isolate and the remaining mutations occurred in single isolates. Mutations E2 T116I and E2 K221R changed the protein surface in contact with the host cell receptors. We could not find positive selected sites in our CHIKV sequences from southern Chiapas. This is the first viral phylogeographic reconstruction in ...Continue Reading

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Citations

Aug 28, 2019·Viruses·Penghua Wang, Rong Zhang

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Datasets Mentioned

BETA
MK240310
MK240311

Methods Mentioned

BETA
reverse transcription PCR
PCR
electron microscopy

Software Mentioned

MEGA
MODEL
TREE
BEAST
Tracer
Ultrafast
MEME
PyMOL OS X
MAFFT
SWISS

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