Genetic Variation Among Isolates of the Web Blight Pathogen of Common Bean Based on PCR-RFLP of the ITS-rDNA Region

Plant Disease
Graciela Godoy-LutzK Powers

Abstract

Variability of 45 isolates of Rhizoctonia solani (teleomorph Thanatephorus cucumeris) causing web blight (WB) of common bean, Phaseolus vulgaris, was examined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the internal transcribed spacer regions (ITS1 and ITS2) and the 5.8S subunit (5.8S) of the nuclear ribosomal DNA repeat (ITS-5.8S-rDNA). Isolates were collected from diseased bean leaves from Argentina, Costa Rica, Cuba, Dominican Republic, Honduras, Panama, and Puerto Rico. These WB isolates belong to AG-1 and AG-2 based on anastomosis reaction. Isolates of AG-1 that cause WB were separated into three distinct groups of RFLP patterns from enzymatic digestion of a 740-bp PCR fragment. Microsclerotia-producing isolates (<1 mm) were differentiated from macrosclerotia-producing isolates (5 to 20 mm) based on PCR-RFLP patterns even though they are placed in the same AG1-1B subgroup by anastomosis reaction. WB isolates of AG-2 were separated into two distinct PCR-RFLP groups as previously reported. AG-1 macrosclerotial-producing isolates were the most virulent, whereas isolates of AG-2 were the least virulent. Genetic variability of the WB pathogen may have influenced the failure or su...Continue Reading

Citations

Oct 23, 2010·Journal of Speech, Language, and Hearing Research : JSLHR·Ewa JacewiczJoseph Salmons
Apr 2, 2014·Journal of Speech, Language, and Hearing Research : JSLHR·Ewa Jacewicz, Robert Allen Fox
Mar 1, 2012·Plant Disease·Nadav NitzanNativ Dudai
Aug 10, 2019·Frontiers in Plant Science·Atena OladzadJulie S Pasche
Feb 21, 2007·Journal of Applied Microbiology·R GroschS H Jabaji-Hare

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