Genetic variation in humoral response to an Escherichia coli O157:H7 vaccine in beef cattle

PloS One
Kara B MarleyMichael G Gonda

Abstract

Individuals often respond differently to the same vaccine; some of this variation may be caused by genetic differences among animals. Our objective was to estimate heritability and identify genomic regions associated with humoral response to an Escherichia coli O157:H7 vaccine in beef cattle. Crossbred beef cattle (n = 651) were vaccinated with a commercially available E. coli O157:H7 vaccine. Serum was collected at time of initial vaccination (d 0), booster (d 21), and d 56 after initial vaccination. Total antibodies specific to siderophore receptor and porin proteins in the vaccine were quantified by enzyme-linked immunosorbent assay. Genomic DNA was isolated from whole blood and genotyped with the bovine GeneSeek Genomic Profiler-High Density 78K or 26K Single Nucleotide Polymorphism BeadChip and imputed to 777,000 SNP genotypes. Heritability was estimated by restricted maximum likelihood (REML) using both 1) pedigree and 2) genomic relationships among individuals. Fixed effects were contemporary group, calf age, sex, principal components from SNP genotype data, and pedigree-derived heterozygosity effects. Additive and dominance effects of SNPs were estimated individually while accounting for contemporary group, sex, and the...Continue Reading

References

Jul 1, 1997·Journal of Animal Science·A Nejati-JavaremiJ P Gibson
Apr 7, 2007·The Pediatric Infectious Disease Journal·Nicola P KleinUNKNOWN Clinical Immunization Safety Assessment Network
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Dec 8, 2015·Journal of Animal Science·W M SnellingE J Pollak

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Methods Mentioned

BETA
genotyping
enzyme-linked immunosorbent assay
ELISA
PCA

Software Mentioned

FindHap
SAS
R package “ qqman ”

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