Genetic variation in the response of mice to xenobiotics in vitro. I. General methodology and response to some model compounds

Toxicology in Vitro : an International Journal Published in Association with BIBRA
M F Festing, T Eydmann

Abstract

A new method for studying genetic variation in the response of laboratory mice to xenobiotics using in vitro cultures of somatic cells has been developed. When resident peritoneal cells from ten mouse strains were cultured with methanol, ethanol, dimethylsulphoxide, sodium butyrate, aspirin or trypan blue, each at two dose levels, significant strain differences in response (total protein) were noted for each xenobiotic except methanol, showing that responses are under genetic control. Strain differences in final protein (growth) were also noted. The two alcohols were least toxic, but aspirin was most toxic to strains with fast-growing cells. Most strain means had a gaussian distribution suggesting polygenic control. However a non-gaussian distribution, possibly due to a single dominant resistance gene in C57BL/6 mice, was seen with the 0.6 mm-butyrate. Differential strain responses to various alternatives to foetal bovine serum were also noted. In another study involving 19 mouse strains, the five strains with the Coh(h) or Coh(i) (strain CBA) genotype ranked 1, 2, 3, 4 and 6 in sensitivity to 2.5 mm-coumarin (P < 0.001) and were significantly more sensitive (49.2 +/- 5.9% of controls) than the 13 strains known to have the Coh(...Continue Reading

References

Nov 1, 1976·The Journal of Heredity·B A Taylor
Apr 1, 1976·The Journal of Hygiene·M E Wallace, F J MacSwiney
Jan 1, 1987·Critical Reviews in Toxicology·M F Festing
Jan 1, 1986·Annual Review of Pharmacology and Toxicology·J P Whitlock
Sep 1, 1983·Clinical Pharmacokinetics·E S Vesell, M B Penno
Sep 1, 1982·Clinical Pharmacokinetics·W Kalow

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Citations

Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M J Arranz, M F Festing
Jan 1, 1987·Differentiation; Research in Biological Diversity·C M NagamineG C Koo
Jan 1, 1987·Differentiation; Research in Biological Diversity·C M NagamineG C Koo
Aug 1, 1996·The Journal of Pathology·R D GoldinS N Wickramasinghe
Jan 1, 1987·Critical Reviews in Toxicology·M F Festing

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