Genetic variation of aldolase from Korean isolates of Plasmodium vivax and its usefulness in serodiagnosis.

Malaria Journal
Jung-Yeon KimHyeong-Woo Lee

Abstract

The malaria aldolase is widely used as rapid diagnostic test (RDT), but the efficacy in aspect of its serological effectiveness in diagnosis is not known. The genetic variation of Korean isolates was analysed and recombinant aldolase was evaluated as a serological antigen in Plasmodium vivax malaria. Genomic DNA was purified and the aldolase gene of P. vivax from 25 patients' blood samples was amplified. The samples came from 5 epidemic areas; Bucheon-si, Gimpo-si, Paju-si of Gyeonggido, Gangwha-gun of Incheon metropolitan city, and Cheorwon of Gangwon-do, South Korea. The antigenicity of the recombinant aldolase was tested by western blot and enzyme-linked immunosorbent assay (ELISA). Sequence analysis of 25 Korean isolates of P. vivax showed that the open reading frame (ORF) of 1,110 nucleotides encoded a deduced protein of 369 amino acids (aa). This ORF showed 100% homology with the P. vivax Sal I strain (XM_00165894) and P. vivax WDK strain (AF247063), 87.4% homology with Plasmodium falciparum (AF179421), 90.6% homology with Plasmodium chabaudi (AF247060), 89.5% homology with Plasmodium vinckei (AF247061), and 96.7% homology with Plasmodium knowlesi. A single nucleotide polymorphism (SNP) at nucleotide 180 (G to A, n = 5) w...Continue Reading

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Citations

Apr 23, 2014·Tropical Parasitology·Ss JeremiahSubhash Chandra Parija
Mar 2, 2016·Expert Opinion on Drug Discovery·Bianca K VerlindenLyn-Marié Birkholtz
Sep 21, 2013·Journal of Medical Microbiology·Joel C Mouatcho, J P Dean Goldring
Oct 6, 2016·PLoS Neglected Tropical Diseases·Yossef AlnasserRobert H Gilman

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Datasets Mentioned

BETA
AF247061

Methods Mentioned

BETA
PCR
electrophoresis
ELISA

Software Mentioned

DNASTAR
Clustal
MegAlign
BLAST
GraphPad
DNASTAR package
EditSeq

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