Genetic variation of chlorella viruses: variable regions localized on the CVK2 genomic DNA

Virology
K NishidaT Yamada

Abstract

A physical map of the Chlorella virus CVK2 genomic DNA has been constructed based on a cosmid contig covering the entire genomic region. By using Southern blot analysis with 22 gene probes, the gene arrangement along the genome was compared between CVK2 and PBCV-1, the prototypic member of Phycodnaviridae, whose genomic sequence is now available. The major rearrangements were (1) an insertion of a 20-kbp region around the left end of CVK2 DNA, (2) a duplication of the gene for major capsid protein in CVK2 DNA, (3) deletions/insertions of some open reading frames, and (4) divergence in the terminal inverted repeat sequences. Despite these changes, extensive colinearity was revealed between most of the genes along the CVK2 and PBCV-1 genomes. These data imply that the Chlorella virus genome has an overall high degree of genomic stability, encompassing specific islands of rearrangements.

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Citations

Mar 7, 2006·Virus Research·David D DuniganJames L Van Etten
Nov 18, 2003·Annual Review of Genetics·James L Van Etten
Nov 4, 2000·Virology·I SugimotoT Yamada
Nov 5, 1999·Annual Review of Microbiology·J L Van Etten, R H Meints
Feb 20, 2004·Virology·Takeru KawasakiTakashi Yamada
Oct 20, 2005·Journal of Bioscience and Bioengineering·T YamadaS Hiramatsu
Nov 14, 2002·Virology·Takeru KawasakiTakashi Yamada
Sep 21, 2021·Microbial Genomics·Heli A M MönttinenRobert P Hirt

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